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Raman microspectros...
Raman microspectroscopy reveals long-term extracellular activity of Chlamydiae
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Haider, Susanne (author)
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Wagner, Michael (author)
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Schmid, Markus C. (author)
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- Sixt, Barbara S. (author)
- Department of Microbial Ecology, University of Vienna,Althanstrasse 14, 1090 Vienna, Austria
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Christian, Jan G. (author)
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Häcker, Georg (author)
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Pichler, Peter (author)
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Mechtler, Karl (author)
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Müller, Albert (author)
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Baranyi, Christian (author)
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Toenshoff, Elena R. (author)
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Montanaro, Jacqueline (author)
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Horn, Matthias (author)
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(creator_code:org_t)
- 2010-06-11
- 2010
- English.
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In: Molecular Microbiology. - : Blackwell Publishing. - 0950-382X .- 1365-2958. ; 77:3, s. 687-700
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- The phylum Chlamydiae consists exclusively of obligate intracellular bacteria. Some of them are formidable pathogens of humans, while others occur as symbionts of amoebae. These genetically intractable bacteria possess a developmental cycle consisting of replicative reticulate bodies and infectious elementary bodies, which are believed to be physiologically inactive. Confocal Raman microspectroscopy was applied to differentiate between reticulate bodies and elementary bodies of Protochlamydia amoebophila and to demonstrate in situ the labelling of this amoeba symbiont after addition of isotope-labelled phenylalanine. Unexpectedly, uptake of this amino acid was also observed for both developmental stages for up to 3 weeks, if incubated extracellularly with labelled phenylalanine, and P. amoebophila remained infective during this period. Furthermore, P. amoebophila energizes its membrane and performs protein synthesis outside of its host. Importantly, amino acid uptake and protein synthesis after extended extracellular incubation could also be demonstrated for the human pathogen Chlamydia trachomatis, which synthesizes stress-related proteins under these conditions as shown by 2-D gel electrophoresis and MALDI-TOF/TOF mass spectrometry. These findings change our perception of chlamydial biology and reveal that host-free analyses possess a previously not recognized potential for direct experimental access to these elusive microorganisms.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Haider, Susanne
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Wagner, Michael
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Schmid, Markus C ...
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Sixt, Barbara S.
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Christian, Jan G ...
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Häcker, Georg
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show more...
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Pichler, Peter
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Mechtler, Karl
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Müller, Albert
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Baranyi, Christi ...
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Toenshoff, Elena ...
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Montanaro, Jacqu ...
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Horn, Matthias
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Molecular Microb ...
- By the university
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Umeå University