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Propidium monoazide...
Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua
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Løvdal, Trond (author)
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Hovda, Maria Befring (author)
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- Björkblom, Benny (author)
- Centre for Organelle Research, Faculty of Science and Technology, University of Stavanger, Stavanger, Norway; The Norwegian Centre for Movement Disorders, Stavanger University Hospital, Stavanger, Norway
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Møller, Simon G. (author)
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(creator_code:org_t)
- Elsevier, 2011
- 2011
- English.
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In: Journal of Microbiological Methods. - : Elsevier. - 0167-7012 .- 1872-8359. ; 85:2, s. 164-169
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Microbiology (medical)
- Molecular Biology
- Microbiology
Publication and Content Type
- ref (subject category)
- art (subject category)
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