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Clinical significan...
Clinical significance of stem cell biomarkers epcam, lgr5 and lgr4 mrna levels in lymph nodes of colon cancer patients
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- AbdelMageed, Manar (author)
- Umeå University,Umeå universitet,Klinisk immunologi,Onkologi,Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
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- Ismail, Hager (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi,Onkologi,Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
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- Ohlsson, Lina (author)
- Umeå University,Umeå universitet,Immunologi/immunkemi
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- Lindmark, Gudrun (author)
- Lund University,Lunds universitet,Kirurgi,Forskargrupper vid Lunds universitet,Surgery,Lund University Research Groups
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- Hammarström, Marie-Louise (author)
- Umeå University,Umeå universitet,Immunologi/immunkemi
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- Hammarström, Sten (author)
- Umeå University,Umeå universitet,Immunologi/immunkemi
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- Sitohy, Basel (author)
- Umeå University,Umeå universitet,Onkologi,Klinisk immunologi
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(creator_code:org_t)
- 2021-12-30
- 2022
- English.
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In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 23:1
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Abstract
Subject headings
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- The significance of cancer stem cells (CSCs) in initiation and progression of colon cancer (CC) has been established. In this study, we investigated the utility of measuring mRNA expression levels of CSC markers EpCAM, LGR5 and LGR4 for predicting survival outcome in surgically treated CC patients. Expression levels were determined in 5 CC cell lines, 66 primary CC tumors and 382 regional lymph nodes of 121 CC patients. Prognostic relevance was determined using Kaplan‐Meier survival and Cox regression analyses. CC patients with lymph nodes expressing high levels of EpCAM, LGR5 or LGR4 (higher than a clinical cutoff of 0.07, 0.06 and 2.558 mRNA cop-ies/18S rRNA unit, respectively) had a decreased mean survival time of 32 months for EpCAM and 42 months for both LGR5 and LGR4 at a 12‐year follow‐up (p = 0.022, p = 0.005 and p = 0.011, respec-tively). Additional patients at risk for recurrence were detected when LGR5 was combined with the biomarkers CXCL17 or CEA plus CXCL16. In conclusion, the study underscores LGR5 as a particularly useful prognostic biomarker and illustrates the strength of combining biomarkers detecting different subpopulations of cancer cells and/or cells in the tumor microenvironment for predicting recurrence.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- CEA
- Colon cancer
- CXCL16
- CXCL17
- EpCAM
- LGR4
- LGR5
- Prognosis
- QRT‐PCR
- Regional lymph nodes
- Stem cell mark-ers
- CEA
- Colon cancer
- CXCL16
- CXCL17
- EpCAM
- LGR4
- LGR5
- Prognosis
- QRT‐PCR
- Regional lymph nodes
- Stem cell mark-ers
Publication and Content Type
- ref (subject category)
- art (subject category)
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