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Divergence of activ...
Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches
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- Xu, Hui (author)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China
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- Li, Zhen (author)
- Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium
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- Jiang, Peng-Fei (author)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China
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- Zhao, Li (author)
- Umeå universitet,Institutionen för ekologi, miljö och geovetenskap,UMFpub
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- Qu, Chang (author)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China
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- Van de Peer, Yves (author)
- Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium; Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria, South Africa
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- Liu, Yan-Jing (author)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China
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- Zeng, Qing-Yin (author)
- State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China
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(creator_code:org_t)
- 2022-02-22
- 2022
- English.
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In: The Plant Journal. - : John Wiley & Sons. - 0960-7412 .- 1365-313X. ; 110:1, s. 129-146
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contributions of active site motifs to the functional divergence of duplicate genes have not been well elucidated. In this study, we identified 41 glutaredoxin (GRX) genes in the Populus trichocarpa genome. GRXs are ubiquitous enzymes in plants that play important roles in developmental and stress tolerance processes. In poplar, GRX genes were divided into four classes based on clear differences in gene structure and expression pattern, subcellular localization, enzymatic activity, and substrate specificity of the encoded proteins. Using site-directed mutagenesis, this study revealed that the divergence of the active site motif among different classes of GRX proteins resulted in substrate switches and thus provided new insights into the molecular evolution of these important plant enzymes.
Subject headings
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- active site motif
- enzymatic specificity
- functional divergence
- gene family
- glutaredoxin
- Populus trichocarpa
Publication and Content Type
- ref (subject category)
- art (subject category)
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