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Structure of SpoT r...
Structure of SpoT reveals evolutionary tuning of catalysis via conformational constraint
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- Tamman, Hedvig (author)
- Cellular and Molecular Microbiology, Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du Triomphe, Brussels, Belgium
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- Ernits, Karin (author)
- Umeå University,Lund University,Lunds universitet,Umeå universitet,Kemiska institutionen,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Experimental Medicine, University of Lund, Lund, Sweden,Molekylär enzymologi,Forskargrupper vid Lunds universitet,Molecular Enzymology,Lund University Research Groups
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- Roghanian, Mohammad (author)
- Umeå University,Lund University,Lunds universitet,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Experimental Medicine, University of Lund, Lund, Sweden; Departement of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark,Molekylär enzymologi,Forskargrupper vid Lunds universitet,Molecular Enzymology,Lund University Research Groups,Copenhagen University Hospital
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- Ainelo, Andres (author)
- Cellular and Molecular Microbiology, Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du Triomphe, Brussels, Belgium
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- Julius, Christina (author)
- Umeå University,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Perrier, Anthony (author)
- Biology of Microorganisms Research Unit, Namur Research Institute for Life Science, University of Namur, Namur, Belgium; Bacterial Cell Cycle and Development, Biology of Microorganisms Research Unit, Namur Research Institute for Life Science, University of Namur, Namur, Belgium
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- Talavera, Ariel (author)
- Cellular and Molecular Microbiology, Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du Triomphe, Brussels, Belgium
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- Ainelo, Hanna (author)
- Cellular and Molecular Microbiology, Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du Triomphe, Brussels, Belgium
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- Dugauquier, Rémy (author)
- Cellular and Molecular Microbiology, Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du Triomphe, Brussels, Belgium; Biology of Microorganisms Research Unit, Namur Research Institute for Life Science, University of Namur, Namur, Belgium
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- Zedek, Safia (author)
- Cellular and Molecular Microbiology, Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du Triomphe, Brussels, Belgium
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- Thureau, Aurelien (author)
- Synchrotron SOLEIL, Saint-Aubin - BP 48, Gif sur Yvette, France
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- Pérez, Javier (author)
- Synchrotron SOLEIL, Saint-Aubin - BP 48, Gif sur Yvette, France
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- Lima-Mendez, Gipsi (author)
- Biology of Microorganisms Research Unit, Namur Research Institute for Life Science, University of Namur, Namur, Belgium
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- Hallez, Régis (author)
- Biology of Microorganisms Research Unit, Namur Research Institute for Life Science, University of Namur, Namur, Belgium; Bacterial Cell Cycle and Development, Biology of Microorganisms Research Unit, Namur Research Institute for Life Science, University of Namur, Namur, Belgium; WELBIO, Brussels, Belgium
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- Atkinson, Gemma C. (author)
- Umeå University,Lund University,Lunds universitet,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Experimental Medicine, University of Lund, Lund, Sweden,Proteinevolution,Forskargrupper vid Lunds universitet,Protein Evolution,Lund University Research Groups
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- Hauryliuk, Vasili, 1980- (author)
- Umeå University,Lund University,Lunds universitet,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Experimental Medicine, University of Lund, Lund, Sweden; University of Tartu, Institute of Technology, Tartu, Estonia,Molekylär enzymologi,Forskargrupper vid Lunds universitet,Molecular Enzymology,Lund University Research Groups
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- Garcia-Pino, Abel (author)
- Cellular and Molecular Microbiology, Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du Triomphe, Brussels, Belgium; WELBIO, Brussels, Belgium
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(creator_code:org_t)
- 2022-12-05
- 2023
- English.
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In: Nature Chemical Biology. - : Springer Nature. - 1552-4450 .- 1552-4469. ; 19, s. 334-345
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Abstract
Subject headings
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- Stringent factors orchestrate bacterial cell reprogramming through increasing the level of the alarmones (p)ppGpp. In Beta- and Gammaproteobacteria, SpoT hydrolyzes (p)ppGpp to counteract the synthetase activity of RelA. However, structural information about how SpoT controls the levels of (p)ppGpp is missing. Here we present the crystal structure of the hydrolase-only SpoT from Acinetobacter baumannii and uncover the mechanism of intramolecular regulation of ‘long’-stringent factors. In contrast to ribosome-associated Rel/RelA that adopt an elongated structure, SpoT assumes a compact τ-shaped structure in which the regulatory domains wrap around a Core subdomain that controls the conformational state of the enzyme. The Core is key to the specialization of long RelA-SpoT homologs toward either synthesis or hydrolysis: the short and structured Core of SpoT stabilizes the τ-state priming the hydrolase domain for (p)ppGpp hydrolysis, whereas the longer, more dynamic Core domain of RelA destabilizes the τ-state priming the monofunctional RelA for efficient (p)ppGpp synthesis. [Figure not available: see fulltext.].
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Tamman, Hedvig
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Ernits, Karin
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Roghanian, Moham ...
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Ainelo, Andres
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Julius, Christin ...
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Perrier, Anthony
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Talavera, Ariel
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Ainelo, Hanna
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Dugauquier, Rémy
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Zedek, Safia
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Thureau, Aurelie ...
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Pérez, Javier
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Lima-Mendez, Gip ...
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Hallez, Régis
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Atkinson, Gemma ...
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Hauryliuk, Vasil ...
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Garcia-Pino, Abe ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
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Nature Chemical ...
- By the university
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Umeå University
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Lund University