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Detection of DNA da...
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
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- Boutet-Robinet, Elisa (author)
- Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, Toulouse, France
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- Haykal, Maria M. (author)
- INSERM U981, LabEx LERMIT, Université Paris 12 Sud, Gustave Roussy Research Center, Department of Molecular Medicine, Villejuif, France
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- Hashim, Saleha (author)
- Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, Toulouse, France
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- Frisan, Teresa (author)
- Umeå universitet,Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)
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- Martin, Océane C.B. (author)
- University of Bordeaux, INSERM, BaRITOn, U1053, Bordeaux, France
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(creator_code:org_t)
- Cell Press, 2021
- 2021
- English.
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In: STAR Protocols. - : Cell Press. - 2666-1667. ; 2:4
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Abstract
Subject headings
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- We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by the alkaline comet assay of colonic mucosal samples. The description of the comet assay protocol follows the international guidelines (Minimum Information for Reporting on the Comet Assay [Moller et al., 2020]). For complete details on the use and execution of this protocol, please refer to Martin et al. (2021).
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Cell isolation
- Cell-based Assays
- Microscopy
Publication and Content Type
- ref (subject category)
- art (subject category)
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