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Hierarchical Cluste...
Hierarchical Clustering and Trajectory Analyses Reveal Viremia-Independent B-Cell Perturbations in HIV-2 Infection
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- Johansson, Emil (author)
- Lund University,Lunds universitet,HIV-1 och HIV-2 värd interaktioner,Forskargrupper vid Lunds universitet,HIV-1 and HIV-2 host interactions,Lund University Research Groups
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- Kerkman, Priscilla (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi,Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, Netherlands
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- Scharf, Lydia (author)
- Karolinska Institute
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- Lindman, Jacob (author)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Szojka, Zsófia I. (author)
- Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine
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- Månsson, Fredrik (author)
- Lund University,Lunds universitet,Klinisk infektionsmedicin,Forskargrupper vid Lunds universitet,Clinical infection medicine,Lund University Research Groups
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- Biague, Antonio (author)
- National Laboratory for Public Health, Bissau, Guinea-Bissau
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- Medstrand, Patrik (author)
- Lund University,Lunds universitet,Klinisk virologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Virology, Malmö,Lund University Research Groups
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- Norrgren, Hans (author)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Buggert, Marcus (author)
- Karolinska Institute,Karolinska Institutet
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- Karlsson, Annika C. (author)
- Karolinska Institute,Karolinska Institutet
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- Forsell, Mattias N. E. (author)
- Umeå University,Umeå universitet,Institutionen för klinisk mikrobiologi
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- Esbjörnsson, Joakim (author)
- Lund University,Lunds universitet,Systemvirologi,Forskargrupper vid Lunds universitet,Systems Virology,Lund University Research Groups,University of Oxford
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- Jansson, Marianne (author)
- Lund University,Lunds universitet,HIV-1 och HIV-2 värd interaktioner,Forskargrupper vid Lunds universitet,HIV-1 and HIV-2 host interactions,Lund University Research Groups
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(creator_code:org_t)
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- 2022-10-06
- 2022
- English.
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In: Cells. - : MDPI. - 2073-4409. ; 11:19
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Abstract
Subject headings
Close
- Time to AIDS in HIV-2 infection is approximately twice as long compared to in HIV-1 infection. Despite reduced viremia, HIV-2-infected individuals display signs of chronic immune activation. In HIV-1-infected individuals, B-cell hyperactivation is driven by continuous antigen exposure. However, the contribution of viremia to B-cell perturbations in HIV-2-infected individuals remains largely unexplored. Here, we used polychromatic flow cytometry, consensus hierarchical clustering and pseudotime trajectory inference to characterize B-cells in HIV-1- or HIV-2-infected and in HIV seronegative individuals. We observed increased frequencies of clusters containing hyperactivated T-bethighCD95highCD27int and proliferating T-bet+CD95highCD27+CD71+ memory B-cells in viremic HIV-1 (p < 0.001 and p < 0.001, respectively), viremic HIV-2 (p < 0.001 and p = 0.014, respectively) and in treatment-naïve aviremic HIV-2 (p = 0.004 and p = 0.020, respectively)-infected individuals, compared to seronegative individuals. In contrast, these expansions were not observed in successfully treated HIV-1-infected individuals. Finally, pseudotime trajectory inference showed that T-bet-expressing hyperactivated and proliferating memory B-cell populations were located at the terminal end of two trajectories, in both HIV-1 and HIV-2 infections. As the treatment-naïve aviremic HIV-2-infected individuals, but not the successfully ART-treated HIV-1-infected individuals, showed B-cell perturbations, our data suggest that aviremic HIV-2-infected individuals would also benefit from antiretroviral treatment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- B-cell phenotype
- CD95
- HIV-1
- HIV-2
- immune perturbations
- T-bet
- viremia
- B-cell phenotype
- CD95
- HIV-1
- HIV-2
- immune perturbations
- T-bet
- viremia
Publication and Content Type
- ref (subject category)
- art (subject category)
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Cells
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Johansson, Emil
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Kerkman, Priscil ...
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Scharf, Lydia
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Lindman, Jacob
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Szojka, Zsófia I ...
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Månsson, Fredrik
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Biague, Antonio
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Medstrand, Patri ...
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Norrgren, Hans
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Buggert, Marcus
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Karlsson, Annika ...
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Forsell, Mattias ...
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Esbjörnsson, Joa ...
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Jansson, Mariann ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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Cells
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Umeå University
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Karolinska Institutet
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Lund University