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Bioprinted schwann ...
Bioprinted schwann and mesenchymal stem cell co-cultures for enhanced spatial control of neurite outgrowth
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- Alakpa, Enateri V. (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Bahrd, Anton (author)
- Umeå universitet,Institutionen för fysik
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- Wiklund, Krister (author)
- Umeå universitet,Institutionen för fysik
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- Andersson, Magnus, 1975- (author)
- Umeå universitet,Institutionen för fysik,The Biophysics and Biophotonics group
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- Novikov, Lev N. (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Ljungberg, Christina, 1966- (author)
- Umeå universitet,Handkirurgi
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- Kelk, Peyman (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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(creator_code:org_t)
- 2023-02-22
- 2023
- English.
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In: Gels. - : MDPI. - 2310-2861. ; 9:3
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https://doi.org/10.3...
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https://umu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- Bioprinting nerve conduits supplemented with glial or stem cells is a promising approach to promote axonal regeneration in the injured nervous system. In this study, we examined the effects of different compositions of bioprinted fibrin hydrogels supplemented with Schwann cells and mesenchymal stem cells (MSCs) on cell viability, production of neurotrophic factors, and neurite outgrowth from adult sensory neurons. To reduce cell damage during bioprinting, we analyzed and optimized the shear stress magnitude and exposure time. The results demonstrated that fibrin hydrogel made from 9 mg/mL of fibrinogen and 50IE/mL of thrombin maintained the gel’s highest stability and cell viability. Gene transcription levels for neurotrophic factors were significantly higher in cultures containing Schwann cells. However, the amount of the secreted neurotrophic factors was similar in all co-cultures with the different ratios of Schwann cells and MSCs. By testing various co-culture combinations, we found that the number of Schwann cells can feasibly be reduced by half and still stimulate guided neurite outgrowth in a 3D-printed fibrin matrix. This study demonstrates that bioprinting can be used to develop nerve conduits with optimized cell compositions to guide axonal regeneration.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- 3D bioprinting
- biosynthetic conduit
- dorsal root ganglion
- mesenchymal stem cells
- nerve regeneration
- Schwann cells
Publication and Content Type
- ref (subject category)
- art (subject category)
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