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- Roberts, Irma NUmeå universitet,Kemiska institutionen,Umeå Plant Science Centre (UPSC) (author)
PsbO degradation by deg proteases under reducing conditions
- Article/chapterEnglish2013
Publisher, publication year, extent ...
- Berlin, Heidelberg :Springer Berlin/Heidelberg,2013
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:umu-208557
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-208557URI
- https://doi.org/10.1007/978-3-642-32034-7_130DOI
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- Language:English
- Summary in:English
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Notes
- DegP/HtrA proteases are ATP-independent serine endopeptidases widely distributed in nearly all organisms. As yet, their physiological role in oxygenic photosynthetic organisms is unclear, although it has been widely speculated that they participate in the photosystem II repair cycle. Here, we investigated the ability of Deg proteases to degrade PsbO according to its redox state. A sample of purified PsbO or photosystem II complex was incubated together with recombinant Deg proteases of Synechocystis sp. PCC 6803 (HhoA, HhoB or HtrA). The reducing media was conferred by the Escherichia coli thioredoxin/thioredoxin reductase system. The results obtained showed that HhoA is able to hydrolyze reduced PsbO while HhoB and HtrA are not. HhoA was active against free PsbO of spinach as well as PsbO of Synechocystis attached to photosystem II, only under reducing conditions. The finding that all three Deg proteases of Synechocystis co-purify with photosystem II supports the hypothesis of PsbO as a substrate for Deg proteases in vivo.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- Deg proteases
- Protein degradation
- PsbO
- Sp. PCC 6803
- Synechocystis
- Thioredoxin
Added entries (persons, corporate bodies, meetings, titles ...)
- Miranda, Hélder,1981-Umeå universitet,Kemiska institutionen,Umeå Plant Science Centre (UPSC)(Swepub:umu)hemi0006 (author)
- Tam, Lam Xuan,1986-Umeå universitet,Kemiska institutionen,Umeå Plant Science Centre (UPSC)(Swepub:umu)xuta0001 (author)
- Kieselbach, ThomasUmeå universitet,Kemiska institutionen,Umeå Plant Science Centre (UPSC)(Swepub:umu)thskih04 (author)
- Funk, ChristianeUmeå universitet,Kemiska institutionen,Umeå Plant Science Centre (UPSC)(Swepub:umu)chefuk04 (author)
- Umeå universitetKemiska institutionen (creator_code:org_t)
Related titles
- In:Photosynthesis research for food, fuel and futureBerlin, Heidelberg : Springer Berlin/Heidelberg, s. 599-60297836423203309783642320347
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