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qRT-PCR analysis of...
qRT-PCR analysis of CEACAM5, KLK6, SLC35D3, MUC2 and POSTN in colon cancer lymph nodes : An improved method for assessment of tumor stage and prognosis
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- Lindmark, Gudrun (author)
- Department of Clinical Sciences, Lund University, Helsingborg, Sweden; Specialistläkarna, Malmö, Sweden,Lund Univ, Sweden; Specialistlakarna, Sweden
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- Olsson, Lina (author)
- HiloProbe AB, Umeå, Sweden
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- Sitohy, Basel (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Onkologi,Umea Univ, Sweden
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- Israelsson, Anne (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Umea Univ, Sweden
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- Blomqvist, Joel (author)
- HiloProbe AB, Umeå, Sweden
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- Kero, Sara (author)
- HiloProbe AB, Umeå, Sweden
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- Roshdy, Tamer (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Onkologi,Department of Molecular Biology, Genetic Engineering, and Biotechnology Research Institute, University of Sadat City, Sadat City, Menoufia, Egypt,Umea Univ, Sweden; Univ Sadat City, Egypt
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- Söderholm, Mattias (author)
- Department of Surgery, Blekinge Hospital, Karlskrona, Sweden,Blekinge Hosp, Sweden
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- Turi, Annamaria (author)
- Department of Clinical Pathology and Cytology, Blekinge Hospital, Karlskrona, Sweden,Blekinge Hosp, Sweden
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- Isaksson, Jessica (author)
- Department of Clinical Pathology and Cytology, Blekinge Hospital, Karlskrona, Sweden,Blekinge Hosp, Sweden
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- Sakari, Thorbjörn (author)
- Department of Surgical Sciences, Uppsala University Hospital, Uppsala, Sweden; Department of Surgery, Gävle Hospital, Gävle, Sweden,Uppsala Univ Hosp, Sweden; Gavle Cent Hosp, Sweden
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- Dooper, Michiel (author)
- Department of Clinical Pathology and Cytology, Gävle Hospital, Gävle, Sweden,Gavle Cent Hosp, Sweden
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- Dafnis, George (author)
- Colorectal Unit, Department of Surgery and Urology, Mälarsjukhuset, Eskilstuna, Sweden
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- Forsberg, Pehr (author)
- Unilabs, Clinical Pathology and Cytology, Mälarsjukhuset, Eskilstuna, Sweden
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- Skovsted, Susanne (author)
- Unit for Surgery, Örnsköldsvik Hospital, Örnsköldsvik, Sweden,Ornskoldsvik Hosp, Sweden
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- Walldén, Maria (author)
- Centrum for Surgery, Sundsvall Hospital, Sundsvall, Sweden,Sundsvall Hosp, Sweden
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- Kung, Chih-Han (author)
- Umeå universitet,Kirurgi,Department of Surgery, Skellefteå Hospital, Skellefteå, Sweden,Umea Univ, Sweden; Skelleftea Hosp, Sweden
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- Rutegård, Martin, 1982- (author)
- Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Kirurgi,Umea Univ, Sweden
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- Nordmyr, Johanna (author)
- Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten,Region Östergötland, Klinisk patologi
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- Muhrbeck, Måns (author)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Kirurgiska kliniken ViN
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- Hammarström, Sten (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Umea Univ, Sweden
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- Hammarström, Marie-Louise (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Umea Univ, Sweden
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(creator_code:org_t)
- John Wiley & Sons, 2024
- 2024
- English.
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In: International Journal of Cancer. - : John Wiley & Sons. - 0020-7136 .- 1097-0215. ; 154:3, s. 573-584
- Related links:
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Abstract
Subject headings
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- One fourth of colorectal cancer patients having curative surgery will relapse of which the majority will die. Lymph node (LN) metastasis is the single most important prognostic factor and a key factor when deciding on postoperative treatment. Presently, LN metastases are identified by histopathological examination, a subjective method analyzing only a small LN volume and giving no information on tumor aggressiveness. To better identify patients at risk of relapse we constructed a qRT-PCR test, ColoNode, that determines levels of CEACAM5, KLK6, SLC35D3, MUC2 and POSTN mRNAs. Combined these biomarkers estimate the tumor cell load and aggressiveness allocating patients to risk categories with low (0, −1), medium (1), high (2) and very high (3) risk of recurrence. Here we present result of a prospective, national multicenter study including 196 colon cancer patients from 8 hospitals. On average, 21 LNs/patient, totally 4698 LNs, were examined by both histopathology and ColoNode. At 3-year follow-up, 36 patients had died from colon cancer or lived with recurrence. ColoNode identified all patients that were identified by histopathology and in addition 9 patients who were undetected by histopathology. Thus, 25% of the patients who recurred were identified by ColoNode only. Multivariate Cox regression analysis proved ColoNode (1, 2, 3 vs 0, −1) as a highly significant risk factor with HR 4.24 [95% confidence interval, 1.42-12.69, P =.01], while pTN-stage (III vs I/II) lost its univariate significance. In conclusion, ColoNode surpassed histopathology by identifying a significantly larger number of patients with future relapse and will be a valuable tool for decisions on postoperative treatment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Keyword
- colon cancer
- ColoNode
- lymph nodes
- prognosis
- tumor markers
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Lindmark, Gudrun
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Olsson, Lina
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Sitohy, Basel
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Israelsson, Anne
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Blomqvist, Joel
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Kero, Sara
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show more...
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Roshdy, Tamer
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Söderholm, Matti ...
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Turi, Annamaria
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Isaksson, Jessic ...
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Sakari, Thorbjör ...
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Dooper, Michiel
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Dafnis, George
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Forsberg, Pehr
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Skovsted, Susann ...
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Walldén, Maria
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Kung, Chih-Han
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Rutegård, Martin ...
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Nordmyr, Johanna
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Muhrbeck, Måns
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Hammarström, Ste ...
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Hammarström, Mar ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
- Articles in the publication
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International Jo ...
- By the university
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Umeå University
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Linköping University