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Efficient clathrin-...
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Becker, MiriamUmeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Molekylär Infektionsmedicin, Sverige (MIMS),Department of Biochemistry, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany
(author)
Efficient clathrin-mediated entry of enteric adenoviruses in human duodenal cells
- Article/chapterEnglish2023
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2023
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LIBRIS-ID:oai:DiVA.org:umu-216662
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-216662URI
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https://doi.org/10.1128/jvi.00770-23DOI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Enteric adenovirus types F40 and 41 (EAdVs) are a leading cause of diarrhea and diarrhea-associated death in young children and have recently been proposed to cause acute hepatitis in children. EAdVs have a unique capsid architecture and exhibit — unlike other human adenoviruses — a relatively strict tropism for gastrointestinal tissues with, to date, understudied infection mechanism and unknown target cells. In this study, we turn to potentially limiting host factors by comparing EAdV entry in cell lines with respiratory and intestinal origin by cellular perturbation, virus particle tracking, and transmission electron microscopy. Our analyses highlight kinetic advantages for EAdVs in duodenal HuTu80 cell infection and reveal a larger fraction of mobile particles, faster virus uptake, and infectious particle entry in intestinal cells. Moreover, EAdVs display a dependence on clathrin- and dynamin-dependent pathways in intestinal cells. Detailed knowledge of virus entry routes and host factor requirements is essential to understanding pathogenesis and developing new countermeasures. Hence, this study provides novel insights into the entry mechanisms of a medically important virus with emerging tropism in a cell line originating from a relevant tissue. IMPORTANCE Enteric adenoviruses have historically been difficult to grow in cell culture, which has resulted in lack of knowledge of host factors and pathways required for infection of these medically relevant viruses. Previous studies in non-intestinal cell lines showed slow infection kinetics and generated comparatively low virus yields compared to other adenovirus types. We suggest duodenum-derived HuTu80 cells as a superior cell line for studies to complement efforts using complex intestinal tissue models. We show that viral host cell factors required for virus entry differ between cell lines from distinct origins and demonstrate the importance of clathrin-mediated endocytosis.
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Conca, Dario ValterUmeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM)(Swepub:umu)daco0028
(author)
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Dorma, NoemiUmeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM)(Swepub:umu)nodo0006
(author)
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Mistry, NiteshUmeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM)(Swepub:umu)mini0002
(author)
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Hahlin, ElinUmeå universitet,Institutionen för klinisk mikrobiologi(Swepub:umu)elpa0031
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Frängsmyr, LarsUmeå universitet,Institutionen för klinisk mikrobiologi(Swepub:umu)lacfrr79
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Bally, MartaUmeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM)(Swepub:umu)maba0261
(author)
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Arnberg, Niklas,1987-Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)(Swepub:umu)niar0001
(author)
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Gerold, Gisa,1979-Umeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Department of Biochemistry, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany(Swepub:umu)gige0003
(author)
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Umeå universitetInstitutionen för klinisk mikrobiologi
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In:Journal of Virology97:100022-538X1098-5514
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Becker, Miriam
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Conca, Dario Val ...
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Dorma, Noemi
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Mistry, Nitesh
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Hahlin, Elin
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Frängsmyr, Lars
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Bally, Marta
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Arnberg, Niklas, ...
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