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The matrix metallop...
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Carriquí-Madroñal, BelénDepartment of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hanover, Germany
(author)
The matrix metalloproteinase ADAM10 supports hepatitis C virus entry and cell-to-cell spread via its sheddase activity
- Article/chapterEnglish2023
Publisher, publication year, extent ...
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Public Library of Science (PLoS),2023
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Numbers
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LIBRIS-ID:oai:DiVA.org:umu-217260
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-217260URI
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https://doi.org/10.1371/journal.ppat.1011759DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Hepatitis C virus (HCV) exploits the four entry factors CD81, scavenger receptor class B type I (SR-BI, also known as SCARB1), occludin, and claudin-1 as well as the co-factor epidermal growth factor receptor (EGFR) to infect human hepatocytes. Here, we report that the disintegrin and matrix metalloproteinase 10 (ADAM10) associates with CD81, SR-BI, and EGFR and acts as HCV host factor. Pharmacological inhibition, siRNA-mediated silencing and genetic ablation of ADAM10 reduced HCV infection. ADAM10 was dispensable for HCV replication but supported HCV entry and cell-to-cell spread. Substrates of the ADAM10 sheddase including epidermal growth factor (EGF) and E-cadherin, which activate EGFR family members, rescued HCV infection of ADAM10 knockout cells. ADAM10 did not influence infection with other enveloped RNA viruses such as alphaviruses and a common cold coronavirus. Collectively, our study reveals a critical role for the sheddase ADAM10 as a HCV host factor, contributing to EGFR family member transactivation and as a consequence to HCV uptake.
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Sheldon, JulieInstitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hanover, Germany
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Duven, MaraDepartment of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hanover, Germany
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Stegmann, CoraDepartment of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hanover, Germany
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Cirksena, KarstenDepartment of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hanover, Germany
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Wyler, EmanuelMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany
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Zapatero-Belinchón, Francisco J.Department of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hanover, Germany; Gladstone Institutes, CA, San Francisco, United States
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Vondran, Florian W. R.Department of General, Visceral and Transplant Surgery, Regenerative Medicine and Experimental Surgery, Hannover Medical School, Hannover, Germany; German Center for Infection Research Partner Site Hannover-Braunschweig Hannover, Germany
(author)
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Gerold, Gisa,1979-Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Avdelningen för virologi,Department of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hanover, Germany; Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hanover, Germany(Swepub:umu)gige0003
(author)
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Department of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hanover, GermanyInstitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hanover, Germany
(creator_code:org_t)
Related titles
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In:PLoS Pathogens: Public Library of Science (PLoS)19:111553-73661553-7374
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