Search: onr:"swepub:oai:DiVA.org:umu-221106" >
Innate immune respo...
Innate immune response to SARS-CoV-2 infection contributes to neuronal damage in human iPSC-derived peripheral neurons
-
- Passos, Vania (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany
-
- Henkel, Lisa M. (author)
- Department of Neurology, Hannover Medical School, Hannover, Germany
-
- Wang, Jiayi (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany
-
show more...
-
- Zapatero-Belinchón, Francisco J. (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,University of Veterinary Medicine Hannover, Foundation, Hannover, Germany; Cluster of Excellence-Resolving Infection Susceptibility (RESIST), Hannover Medical School, Hannover, Germany
-
- Möller, Rebecca (author)
- University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
-
- Sun, Guorong (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany
-
- Waltl, Inken (author)
- Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, A Joint Venture between the Helmholtz Centre for Infection Research and the Hannover Medical School, Hannover, Germany
-
- Schneider, Talia (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany
-
- Wachs, Amelie (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany
-
- Ritter, Birgit (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany
-
- Kropp, Kai A. (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany
-
- Zhu, Shuyong (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany
-
- Deleidi, Michela (author)
- Center of Neurology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany
-
- Kalinke, Ulrich (author)
- Cluster of Excellence-Resolving Infection Susceptibility (RESIST), Hannover Medical School, Hannover, Germany; Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, A Joint Venture between the Helmholtz Centre for Infection Research and the Hannover Medical School, Hannover, Germany
-
- Schulz, Thomas F. (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany; Cluster of Excellence-Resolving Infection Susceptibility (RESIST), Hannover Medical School, Hannover, Germany
-
- Höglinger, Günter (author)
- Department of Neurology, Hannover Medical School, Hannover, Germany; Cluster of Excellence-Resolving Infection Susceptibility (RESIST), Hannover Medical School, Hannover, Germany
-
- Gerold, Gisa, 1979- (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),University of Veterinary Medicine Hannover, Foundation, Hannover, Germany; Cluster of Excellence-Resolving Infection Susceptibility (RESIST), Hannover Medical School, Hannover, Germany
-
- Wegner, Florian (author)
- Department of Neurology, Hannover Medical School, Hannover, Germany
-
- Viejo-Borbolla, Abel (author)
- Hannover Medical School, Institute of Virology, Hannover, Germany; Cluster of Excellence-Resolving Infection Susceptibility (RESIST), Hannover Medical School, Hannover, Germany
-
show less...
-
(creator_code:org_t)
- John Wiley & Sons, 2024
- 2024
- English.
-
In: Journal of Medical Virology. - : John Wiley & Sons. - 0146-6615 .- 1096-9071. ; 96:2
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://umu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Severe acute respiratory coronavirus 2 (SARS-CoV-2) causes neurological disease in the peripheral and central nervous system (PNS and CNS, respectively) of some patients. It is not clear whether SARS-CoV-2 infection or the subsequent immune response are the key factors that cause neurological disease. Here, we addressed this question by infecting human induced pluripotent stem cell-derived CNS and PNS neurons with SARS-CoV-2. SARS-CoV-2 infected a low number of CNS neurons and did not elicit a robust innate immune response. On the contrary, SARS-CoV-2 infected a higher number of PNS neurons. This resulted in expression of interferon (IFN) λ1, several IFN-stimulated genes and proinflammatory cytokines. The PNS neurons also displayed alterations characteristic of neuronal damage, as increased levels of sterile alpha and Toll/interleukin receptor motif-containing protein 1, amyloid precursor protein and α-synuclein, and lower levels of cytoskeletal proteins. Interestingly, blockade of the Janus kinase and signal transducer and activator of transcription pathway by Ruxolitinib did not increase SARS-CoV-2 infection, but reduced neuronal damage, suggesting that an exacerbated neuronal innate immune response contributes to pathogenesis in the PNS. Our results provide a basis to study coronavirus disease 2019 (COVID-19) related neuronal pathology and to test future preventive or therapeutic strategies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- interferon
- iPSC-derived peripheral neurons
- JAK/STAT
- neuronal damage
- SARM1
- SARS-CoV-2
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Passos, Vania
-
Henkel, Lisa M.
-
Wang, Jiayi
-
Zapatero-Belinch ...
-
Möller, Rebecca
-
Sun, Guorong
-
show more...
-
Waltl, Inken
-
Schneider, Talia
-
Wachs, Amelie
-
Ritter, Birgit
-
Kropp, Kai A.
-
Zhu, Shuyong
-
Deleidi, Michela
-
Kalinke, Ulrich
-
Schulz, Thomas F ...
-
Höglinger, Günte ...
-
Gerold, Gisa, 19 ...
-
Wegner, Florian
-
Viejo-Borbolla, ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Neurosciences
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Infectious Medic ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Immunology in th ...
- Articles in the publication
-
Journal of Medic ...
- By the university
-
Umeå University