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Successful DNA ampl...
Successful DNA amplification of DNA from non-destructive buccal swabbing in Vespertilionid and Rhinolophid bats
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- Hughes, Morgan (author)
- Umeå universitet,Institutionen för ekologi, miljö och geovetenskap,Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, United Kingdom
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- Brown, Scott K. (author)
- School of Biological Sciences, University of Exeter, Exeter, United Kingdom
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- Martin, Rémi (author)
- Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, United Kingdom
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- Young, Christopher H. (author)
- Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, United Kingdom
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- Maddock, Simon (author)
- Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, United Kingdom; Department of Life Sciences, The Natural History Museum, London, United Kingdom; Island Biodiversity and Conservation Centre, University of Seychelles, Mahé, Seychelles; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom
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(creator_code:org_t)
- Springer Nature, 2024
- 2024
- English.
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In: Conservation Genetics Resources. - : Springer Nature. - 1877-7252 .- 1877-7260. ; 16:2, s. 199-202
- Related links:
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https://umu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- Acquiring DNA from wild bats (Mammalia: Chiroptera) is typically undertaken utilizing highly invasive (but non-lethal) sampling techniques comprising wing biopsies and occasional blood samples. While non-invasive sampling is possible through the extraction of DNA from faecal samples, it is not always possible to acquire samples from individual bats whilst conducting fieldwork, and as such, this method is primarily applicable to roost occupancy identification. Similarly, wing swabbing is liable to cross-contamination from roost mates. Here we present the first use of oral (buccal) swabbing for successful, species-resolution DNA sequencing of Vespertilionidae and Rhinolophidae in 10 bat species (nine Vespertilionidae and one Rhinolophidae) from the UK.
Subject headings
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Zoologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Zoology (hsv//eng)
Keyword
- Buccal swabbing
- Conservation
- Population genetics
- Sampling techniques
Publication and Content Type
- ref (subject category)
- art (subject category)
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