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A history of repeat...
A history of repeated antibiotic usage leads to microbiota-dependent mucus defects
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- Krigul, Kertu Liis (author)
- Estonian Genome Centre, Institute of Genomics, University of Tartu, Tartu, Estonia
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- Feeney, Rachel H. (author)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Wongkuna, Supapit (author)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Aasmets, Oliver (author)
- Estonian Genome Centre, Institute of Genomics, University of Tartu, Tartu, Estonia
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- Holmberg, Sandra (author)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Andreson, Reidar (author)
- Estonian Genome Centre, Institute of Genomics, University of Tartu, Tartu, Estonia; Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
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- Puértolas Balint, Fabiola, 1993- (author)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Pantiukh, Kateryna (author)
- Estonian Genome Centre, Institute of Genomics, University of Tartu, Tartu, Estonia
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- Sootak, Linda (author)
- Estonian Genome Centre, Institute of Genomics, University of Tartu, Tartu, Estonia; Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
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- Org, Tõnis (author)
- Estonian Genome Centre, Institute of Genomics, University of Tartu, Tartu, Estonia; Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
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- Tenson, Tanel (author)
- Institute of Technology, University of Tartu, Tartu, Estonia
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- Org, Elin (author)
- Estonian Genome Centre, Institute of Genomics, University of Tartu, Tartu, Estonia
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- Schröder, Björn O. (author)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Medicinska fakulteten)
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(creator_code:org_t)
- Taylor & Francis, 2024
- 2024
- English.
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In: Gut microbes. - : Taylor & Francis. - 1949-0976 .- 1949-0984. ; 16:1
- Related links:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Recent evidence indicates that repeated antibiotic usage lowers microbial diversity and ultimately changes the gut microbiota community. However, the physiological effects of repeated–but not recent–antibiotic usage on microbiota-mediated mucosal barrier function are largely unknown. By selecting human individuals from the deeply phenotyped Estonian Microbiome Cohort (EstMB), we here utilized human-to-mouse fecal microbiota transplantation to explore long-term impacts of repeated antibiotic use on intestinal mucus function. While a healthy mucus layer protects the intestinal epithelium against infection and inflammation, using ex vivo mucus function analyses of viable colonic tissue explants, we show that microbiota from humans with a history of repeated antibiotic use causes reduced mucus growth rate and increased mucus penetrability compared to healthy controls in the transplanted mice. Moreover, shotgun metagenomic sequencing identified a significantly altered microbiota composition in the antibiotic-shaped microbial community, with known mucus-utilizing bacteria, including Akkermansia muciniphila and Bacteroides fragilis, dominating in the gut. The altered microbiota composition was further characterized by a distinct metabolite profile, which may be caused by differential mucus degradation capacity. Consequently, our proof-of-concept study suggests that long-term antibiotic use in humans can result in an altered microbial community that has reduced capacity to maintain proper mucus function in the gut.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Akkermansia
- Antibiotics
- colonic mucosa
- fecal microbiota transplantation
- gut microbiome
- intestinal barrier
- mucus
- short-chain fatty acids
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Krigul, Kertu Li ...
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Feeney, Rachel H ...
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Wongkuna, Supapi ...
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Aasmets, Oliver
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Holmberg, Sandra
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Andreson, Reidar
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show more...
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Puértolas Balint ...
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Pantiukh, Katery ...
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Sootak, Linda
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Org, Tõnis
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Tenson, Tanel
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Org, Elin
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Schröder, Björn ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Gut microbes
- By the university
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Umeå University