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- Alanentalo, TomasUmeå universitet,Umeå centrum för molekylär medicin (UCMM) (author)
High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas
- Article/chapterEnglish2008
Publisher, publication year, extent ...
- Bellingham, WA :SPIE--the International Society for Optical Engineering,2008
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Numbers
- LIBRIS-ID:oai:DiVA.org:umu-23351
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-23351URI
- https://doi.org/10.1117/1.3000430DOI
Supplementary language notes
- Language:English
- Summary in:English
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Notes
- A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required.
Subject headings and genre
- biological organs
- biomedical optical imaging
- cellular biophysics
- diseases
- image resolution
- laser applications in medicine
- molecular biophysics
- optical tomography
- optical projection tomography
- confocal microscopy
- pancreas
- microbiopsy
- insulitis
- diabetes
Added entries (persons, corporate bodies, meetings, titles ...)
- Lorén, Christina EUmeå universitet,Umeå centrum för molekylär medicin (UCMM) (author)
- Larefalk, AsaUmeå universitet,Medicinsk och klinisk genetik(Swepub:umu)asla0002 (author)
- Sharpe, James (author)
- Holmberg, DanUmeå universitet,Medicinsk och klinisk genetik(Swepub:umu)daho0001 (author)
- Ahlgren, UlfUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)ulah0001 (author)
- Umeå universitetUmeå centrum för molekylär medicin (UCMM) (creator_code:org_t)
Related titles
- In:Journal of Biomedical OpticsBellingham, WA : SPIE--the International Society for Optical Engineering13:5, s. 054070-1083-36681560-2281
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