Search: onr:"swepub:oai:DiVA.org:umu-23351" >
High-resolution thr...
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Alanentalo, TomasUmeå universitet,Umeå centrum för molekylär medicin (UCMM)
(author)
High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas
- Article/chapterEnglish2008
Publisher, publication year, extent ...
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Bellingham, WA :SPIE--the International Society for Optical Engineering,2008
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-23351
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-23351URI
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https://doi.org/10.1117/1.3000430DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required.
Subject headings and genre
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biological organs
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biomedical optical imaging
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cellular biophysics
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diseases
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image resolution
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laser applications in medicine
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molecular biophysics
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optical tomography
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optical projection tomography
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confocal microscopy
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pancreas
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microbiopsy
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insulitis
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diabetes
Added entries (persons, corporate bodies, meetings, titles ...)
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Lorén, Christina EUmeå universitet,Umeå centrum för molekylär medicin (UCMM)
(author)
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Larefalk, AsaUmeå universitet,Medicinsk och klinisk genetik(Swepub:umu)asla0002
(author)
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Sharpe, James
(author)
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Holmberg, DanUmeå universitet,Medicinsk och klinisk genetik(Swepub:umu)daho0001
(author)
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Ahlgren, UlfUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)ulah0001
(author)
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Umeå universitetUmeå centrum för molekylär medicin (UCMM)
(creator_code:org_t)
Related titles
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In:Journal of Biomedical OpticsBellingham, WA : SPIE--the International Society for Optical Engineering13:5, s. 054070-1083-36681560-2281
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