Chronic intermittent L-DOPA treatment induces changes in dopamine release

Lundblad, MartinLund University,Lunds universitet,Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Basala gangliernas patofysiologi,Forskargrupper vid Lunds universitet,Basal Ganglia Pathophysiology,Lund University Research Groups (author)

3,4-Dihydroxyphenyl-l-alanine (l-DOPA)-induced dyskinesia often develops as a side effect of chronic l-DOPA therapy. This study was undertaken to investigate dopamine (DA) release upon l-DOPA treatment. Chronoamperometric measurements were performed in unilaterally DA-depleted rats, chronically treated with l-DOPA, resulting in dyskinetic and non-dyskinetic animals. Normal and lesioned l-DOPA naïve animals were used as controls. Potassium-evoked DA releases were significantly reduced in intact sides of animals undertaken chronic l-DOPA treatment, independent on dyskinetic behavior. Acute l-DOPA further attenuated the amplitude of the DA release in the control sides. In DA-depleted striata, no difference was found in potassium-evoked DA releases, and acute l-DOPA did not affect the amplitude. While immunoreactivity to serotonin uptake transporter was higher in lesioned striata of animals displaying dyskinetic behavior, no correlation could be documented between serotonin transporter-positive nerve fiber density and the amplitude of released DA. In conclusions, the amplitude of potassium-evoked DA release is attenuated in intact striatum after chronic intermittent l-DOPA treatment. No change in amplitude was found in DA-denervated sides of either dyskinetic or non-dyskinetic animals, while release kinetics were changed. This indicates the importance of studying DA release dynamics for the understanding of both beneficial and adverse effects of l-DOPA replacement therapy.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
3
4-dihydroxyphenyl-l-alanine
3
4-dihydroxyphenyl-l-alanine induced dyskinesia
5-hydroxytryptamine or serotonin
chronoamperometry
dopamine
serotonin transporter
MEDICINE
MEDICIN
chronoamperometry
dopamine
serotonin transporter
5-hydroxytryptamine or serotonin
dyskinesia
4-dihydroxyphenyl-l-alanine induced
3
4-dihydroxyphenyl-l-alanine

af Bjerkén, SaraUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB)(Swepub:umu)saaafn99 (author)
Cenci, Angela M. (author)
Pomerleau, Francois (author)
Gerhardt, Greg A. (author)
Strömberg, IngridUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB)(Swepub:umu)inst0001 (author)
Cenci Nilsson, AngelaLund University,Lunds universitet,Basala gangliernas patofysiologi,Forskargrupper vid Lunds universitet,Basal Ganglia Pathophysiology,Lund University Research Groups(Swepub:lu)mphy-ace (author)
Umeå universitetInstitutionen för integrativ medicinsk biologi (IMB) (creator_code:org_t)

By the author/editor: Lundblad, Martin; af Bjerkén, Sara; Cenci, Angela M.; Pomerleau, Franc ...; Gerhardt, Greg A ...; Strömberg, Ingri ...; show more...; Cenci Nilsson, A ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Neurosciences

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