onr:"swepub:oai:DiVA.org:umu-3217"
Search: onr:"swepub:oai:DiVA.org:umu-3217" > Direct repeat-media...
- 1 of 1
- Previous record
- Next record
- To hitlist
Direct repeat-mediated deletion of a type IV pilin gene results in major virulence attenuation of Francisella tularensis
-
- Forslund, Anna-Lena, 1964- (author)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)
- Kuoppa, Kerstin (author)
- Svensson, Kerstin (author)
- show more...
- Johansson, Anders (author)
- Byström, Mona (author)
- Oyston, Petra C. F. (author)
- Michell, Stephen L. (author)
- Titball, Richard W. (author)
- Noppa, Laila (author)
- Frithz-Lindsten, Elisabet (author)
- Forsman, Mats (author)
- show less...
- (creator_code:org_t)
- Wiley-Blackwell, 2006
- 2006
- English.
- In: Molecular Microbiology. - : Wiley-Blackwell. - 0950-382X .- 1365-2958. ; 59:6, s. 1818-1830
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Francisella tularensis, the causative agent of tularaemia, is a highly infectious and virulent intracellular pathogen. There are two main human pathogenic subspecies, Francisella tularensis ssp. tularensis (type A), and Francisella tularensis ssp. holarctica (type B). So far, knowledge regarding key virulence determinants is limited but it is clear that intracellular survival and multiplication is one major virulence strategy of Francisella. In addition, genome sequencing has revealed the presence of genes encoding type IV pili (Tfp). One genomic region encoding three proteins with signatures typical for type IV pilins contained two 120 bp direct repeats. Here we establish that repeat-mediated loss of one of the putative pilin genes in a type B strain results in severe virulence attenuation in mice infected by subcutaneous route. Complementation of the mutant by introduction of the pilin gene in cis resulted in complete restoration of virulence. The level of attenuation was similar to that of the live vaccine strain and this strain was also found to lack the pilin gene as result of a similar deletion event mediated by the direct repeats. Presence of the pilin had no major effect on the ability to interact, survive and multiply inside macrophage-like cell lines. Importantly, the pilin-negative strain was impaired in its ability to spread from the initial site of infection to the spleen. Our findings indicate that this putative pilin is critical for Francisella infections that occur via peripheral routes.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Molecular Microbiology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Forslund, Anna-L ...
- Kuoppa, Kerstin
- Svensson, Kersti ...
- Salomonsson, Eme ...
- Johansson, Ander ...
- Byström, Mona
- show more...
- Oyston, Petra C. ...
- Michell, Stephen ...
- Titball, Richard ...
- Noppa, Laila
- Frithz-Lindsten, ...
- Forsman, Mats
- Forsberg, Åke, 1 ...
- show less...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Biochemistry and ...
- Articles in the publication
- Molecular Microb ...
- By the university
- Umeå University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
