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Two intramolecular ...
Two intramolecular isopeptide bonds are identified in the crystal structure of the Streptococcus gordonii SspB c-terminal domain
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- Forsgren, Nina, 1979- (author)
- Umeå universitet,Kariologi,Karina Persson
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Lamont, Richard J (author)
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- Persson, Karina (author)
- Umeå universitet,Kariologi
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(creator_code:org_t)
- Academic Press, 2010
- 2010
- English.
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In: Journal of Molecular Biology. - : Academic Press. - 0022-2836 .- 1089-8638. ; 397:3, s. 740-751
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Streptococcus gordonii is a primary colonizer and is involved in the formation of dental plaque. This bacterium expresses several surface proteins. One of them is the adhesin SspB, which is a member of the Antigen I/II family of proteins. SspB is a large multi-domain protein that has interactions with surface molecules on other bacteria and on host cells, and is thus a key factor in the formation of biofilms. Here, we report the crystal structure of a truncated form of the SspB C-terminal domain, solved by single-wavelength anomalous dispersion to 1.5Å resolution. The structure represents the first of a C-terminal domain from a streptococcal Antigen I/II protein and is comprised of two structurally related β-sandwich domains, C2 and C3, both with a Ca2+ bound in equivalent positions. In each of the domains, a covalent isopeptide bond is observed between a lysine and an asparagine, a feature that is believed to be a common stabilization mechanism in Gram-positive surface proteins. S. gordonii biofilms contain attachment sites for the periodontal pathogen Porphyromonas gingivalis and the SspB C-terminal domain has been shown to have one such recognition motif, the SspB adherence region. The motif protrudes from the protein, and serves as a handle for attachment. The structure suggests several additional putative binding surfaces, and other binding clefts may be created when the fulllength protein is folded.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- crystal structure
- X-ray crystallography
- protein structure
- surface adhesin
- isopeptide bond
- ODONTOLOGY
- ODONTOLOGI
- Structural biology
- Strukturbiologi
- Biochemistry
- Biokemi
- Biochemistry
- biokemi
- odontologi
- Odontology
Publication and Content Type
- ref (subject category)
- art (subject category)
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