Cadmium-induced decrement of the LH receptor expression and cAMP levels in the testis of rats

Gunnarsson, DavidUmeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet) (author)

Cadmium (Cd) is a widespread environmental pollutant, characterized by its ability to affect various organs. Adverse effect of Cd on the testis including decreased testosterone production are well-known phenomena, but the cellular events explaining these effects have not yet been established. In the present study the initial steps of gonadotropin mediated testosterone biosynthesis were examined in vivo in rats, in relation to Cd dose and time after injection. In the dose–response experiment Male Sprague–Dawley rats received a single subcutaneous (sc) injection of CdCl2 (1, 5 or 10 μmol/kg body weight) and were sacrificed 48 h after injection. A statistically significant decrease in luteinizing hormone (LH) receptor mRNA level in the testicular tissue was demonstrated at the highest dose (10 μmol/kg). In the temporal–response experiment rats were given 10 μmol/kg of CdCl2 sc and sacrificed 0.48, 4.8, 48 or 144 h after injection. LH receptor mRNA levels as well as cyclic adenosine monophosphate (cAMP) levels were found to be significantly lowered at 48 and 144 h. These observations of the mechanisms whereby Cd exerts its effect on the initial steps of testosterone biosynthesis are the first from in vivo experiments.

Cadmium; Luteinizing hormone receptor; Cyclic adenosine monophosphate system; Steroidogenesis; Rat; Testis

Nordberg, GunnarUmeå universitet,Institutionen för folkhälsa och klinisk medicin(Swepub:umu)guno0001 (author)
Lundgren, PerUmeå universitet,Institutionen för klinisk vetenskap(Swepub:umu)pelu0003 (author)
Selstam, GunnarUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)guse0001 (author)
Umeå universitetInstitutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet) (creator_code:org_t)

By the author/editor: Gunnarsson, Davi ...; Nordberg, Gunnar; Lundgren, Per; Selstam, Gunnar

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