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Human extraocular m...
Human extraocular muscles in ALS
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- Ahmadi, Mahboobah (author)
- Umeå universitet,Anatomi,Oftalmiatrik
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- Liu, Jing-Xia (author)
- Umeå universitet,Anatomi
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- Brännström, Thomas (author)
- Umeå universitet,Patologi
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- Andersen, Peter M (author)
- Umeå universitet,Neurologi
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- Stål, Per (author)
- Umeå universitet,Anatomi
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- Pedrosa-Domellöf, Fatima (author)
- Umeå universitet,Oftalmiatrik
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(creator_code:org_t)
- Association for Research in Vision and Ophthalmology (ARVO), 2010
- 2010
- English.
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In: Investigative Ophthalmology and Visual Science. - : Association for Research in Vision and Ophthalmology (ARVO). - 0146-0404 .- 1552-5783. ; 51:7, s. 3494-3501
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- PURPOSE. To investigate the general morphology, fiber type content, and myosin heavy chain (MyHC) composition of extraocular muscles (EOMs) from postmortem donors with amyotrophic lateral sclerosis (ALS) and to evaluate whether EOMs are affected or truly spared in this disease. METHODS. EOM and limb muscle samples obtained at autopsy from ALS donors and EOM samples from four control donors were processed for immunohistochemistry with monoclonal antibodies against distinct MyHC isoforms and analyzed by SDS-PAGE. In addition, hematoxylin and eosin staining and nicotinamide tetrazolium reductase (NADH-TR) activity were studied. RESULTS. Wide heterogeneity was observed in the appearance of the different EOMs from each single donor and between donors, irrespective of ALS type or onset. Pathologic morphologic findings in ALS EOMs included presence of atrophic and hypertrophic fibers, either clustered in groups or scattered; increased amounts of connective tissue; and areas of fatty replacement. The population of fibers stained with anti-MyHCslow tonic was smaller than that of MyHCIpositive fibers and was mostly located in the orbital layer in most of the ALS EOM samples, whereas an identical staining pattern for both fiber populations was observed in the control specimens. MyHCembryonic was notably absent from the ALS EOMs. CONCLUSIONS. The EOMs showed signs of involvement with altered fiber type composition, contractile protein content, and cellular architecture. However, when compared to the limb muscles, the EOMs were remarkably preserved. EOMs are a useful model for the study of the pathophysiology of ALS.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oftalmologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Ophthalmology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Keyword
- ALS
- human extraocular muscles
- Ophtalmiatrics
- Oftalmiatrik
- Neurology
- Neurologi
- neurologi
- Neurology
- oftalmiatrik
- Ophtalmology
- patologi
- Pathology
Publication and Content Type
- ref (subject category)
- art (subject category)
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