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Dominant expression...
Dominant expression and distribution of oestrogen receptor beta over oestrogen receptor alpha in the human corpus luteum
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- Hosokawa, K (author)
- Umeå universitet,Obstetrik och gynekologi
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- Ottander, Ulrika (author)
- Umeå universitet,Obstetrik och gynekologi
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- Wahlberg, P (author)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Ny, Tor (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Cajander, S (author)
- Umeå universitet,Patologi
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- Olofsson, Jan I (author)
- Umeå universitet,Obstetrik och gynekologi
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(creator_code:org_t)
- Oxford University Press (OUP), 2001
- 2001
- English.
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In: Molecular human reproduction. - : Oxford University Press (OUP). - 1360-9947 .- 1460-2407. ; 7:2, s. 137-145
- Related links:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- To investigate the potential importance of oestrogen as a local regulator of human corpus luteum function, the mRNA expression pattern and cellular localization of oestrogen receptors (ERs), ER-alpha and ER-beta, were studied in corpora lutea grouped according to age, where days 2-5 post-LH rise were designated as the early luteal phase, days 6-10 as mid-luteal and days 11-14 as the late luteal phase respectively. Northern blot analysis using an ER-beta probe in samples from whole ovarian tissue and isolated corpora lutea, revealed a major band at 7.5 kb and several minor bands between 4-10 kb, while no signals for ER-alpha mRNA were obtained. However, using a semi-quantitative reverse transcription-polymerase chain reaction followed by Southern blotting, ER-beta mRNA levels were found to be 63% lower (P: < 0.05, n = 39) in the mid-luteal phase compared with the early luteal phase, while ER-alpha mRNA expression showed no statistical differences between the different age groups. Using in-situ hybridization, ER-beta mRNA expression was localized to the steroidogenic luteal cells as well as perivascular cells and fibroblasts in the corpus luteum. Immunohistochemistry confirmed the localization of ER-beta protein, but no clear staining of luteal cells was found using antibodies against ER-alpha. Collectively, the findings of low to moderate expression of ER-beta mRNA and protein in the steroidogenic cells, and also in vascular endothelial cells of the corpus luteum, as opposed to diminutive amounts of ER-alpha mRNA, suggest that oestrogen activity is primarily transduced via ER-beta in the human corpus luteum.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
Keyword
- 17β-oestradiol
- human corpus luteum
- oestrogen receptors
- steroids
Publication and Content Type
- ref (subject category)
- art (subject category)
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