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Dominant expression...
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Hosokawa, KUmeå universitet,Obstetrik och gynekologi
(author)
Dominant expression and distribution of oestrogen receptor beta over oestrogen receptor alpha in the human corpus luteum
- Article/chapterEnglish2001
Publisher, publication year, extent ...
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Oxford University Press (OUP),2001
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-41642
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-41642URI
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https://doi.org/10.1093/molehr/7.2.137DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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To investigate the potential importance of oestrogen as a local regulator of human corpus luteum function, the mRNA expression pattern and cellular localization of oestrogen receptors (ERs), ER-alpha and ER-beta, were studied in corpora lutea grouped according to age, where days 2-5 post-LH rise were designated as the early luteal phase, days 6-10 as mid-luteal and days 11-14 as the late luteal phase respectively. Northern blot analysis using an ER-beta probe in samples from whole ovarian tissue and isolated corpora lutea, revealed a major band at 7.5 kb and several minor bands between 4-10 kb, while no signals for ER-alpha mRNA were obtained. However, using a semi-quantitative reverse transcription-polymerase chain reaction followed by Southern blotting, ER-beta mRNA levels were found to be 63% lower (P: < 0.05, n = 39) in the mid-luteal phase compared with the early luteal phase, while ER-alpha mRNA expression showed no statistical differences between the different age groups. Using in-situ hybridization, ER-beta mRNA expression was localized to the steroidogenic luteal cells as well as perivascular cells and fibroblasts in the corpus luteum. Immunohistochemistry confirmed the localization of ER-beta protein, but no clear staining of luteal cells was found using antibodies against ER-alpha. Collectively, the findings of low to moderate expression of ER-beta mRNA and protein in the steroidogenic cells, and also in vascular endothelial cells of the corpus luteum, as opposed to diminutive amounts of ER-alpha mRNA, suggest that oestrogen activity is primarily transduced via ER-beta in the human corpus luteum.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Ottander, UlrikaUmeå universitet,Obstetrik och gynekologi(Swepub:umu)ulaotr95
(author)
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Wahlberg, PUmeå universitet,Institutionen för medicinsk biovetenskap
(author)
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Ny, TorUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)tony0001
(author)
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Cajander, SUmeå universitet,Patologi
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Olofsson, Jan IUmeå universitet,Obstetrik och gynekologi
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Umeå universitetObstetrik och gynekologi
(creator_code:org_t)
Related titles
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In:Molecular human reproduction: Oxford University Press (OUP)7:2, s. 137-1451360-99471460-2407
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