Differential ability of novel attenuated targeted deletion mutants of Francisella tularensis subspecies tularensis strain SCHU S4 to protect mice against aerosol challenge with virulent bacteria: effects of host background and route of immunization

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:DiVA.org:umu-42613" > Differential abilit...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Conlan, J WayneNRC, Kanada (author)

Differential ability of novel attenuated targeted deletion mutants of Francisella tularensis subspecies tularensis strain SCHU S4 to protect mice against aerosol challenge with virulent bacteria : effects of host background and route of immunization

Article/chapterEnglish2010

Publisher, publication year, extent ...

Elsevier BV,2010
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:umu-42613
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-42613URI
https://doi.org/10.1016/j.vaccine.2009.12.001DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Francisella tularensis subspecies tularensis is a highly virulent facultative intracellular pathogen of humans and a potential biological weapon. A live vaccine strain, F. tularensis LVS, was developed more than 50 years ago by pragmatic attenuation of a strain of the less virulent holarctica subspecies. LVS was demonstrated to be highly effective in human volunteers who were exposed to intradermal challenge with fully virulent subsp. tularensis, but was less effective against aerosol exposure. LVS faces regulatory hurdles that to date have prevented its licensure for general use. Therefore, a better defined and more effective vaccine is being sought. To this end we have created gene deletion mutants in the virulent subsp. tularensis strain and tested them for their ability to elicit a protective immune response against systemic or aerosol challenge with the highly virulent wild-type subsp. tularensis strain, SCHU S4. Both oral and intradermal (ID) primary vaccination routes were assessed in BALB/c and C3H/HeN mice as was oral boosting. One SCHU S4 mutant missing the heat shock gene, clpB, was significantly more attenuated than LVS whereas a double deletion mutant missing genes FTT0918 and capB was as attenuated as LVS. In general mice immunized with SCHU S4DeltaclpB were significantly better protected against aerosol challenge than mice immunized with LVS. A single ID immunization of BALB/c mice with SCHU S4DeltaclpB was at least as effective as any other regimen examined. Mice immunized with SCHU S4Delta0918DeltacapB were generally protected to a similar degree as mice immunized with LVS. A preliminary examination of immune responses to vaccination with LVS, SCHU S4DeltaclpB, or SCHU S4Delta0918DeltacapB provided no obvious correlate to their relative efficacies.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
Francisella tularensis; Mutants; Vaccines; Aerosol

Added entries (persons, corporate bodies, meetings, titles ...)

Shen, Hua (author)
Golovliov, Igor,1958-Umeå universitet,Klinisk bakteriologi,Sjöstedt ; Arcum(Swepub:umu)igrgov98 (author)
Zingmark, Carl,1975-Umeå universitet,Klinisk bakteriologi,Sjöstedt(Swepub:umu)cazi0001 (author)
Oyston, Petra CF (author)
Chen, Wangxue (author)
House, Robert V (author)
Sjöstedt, Anders,1957-Umeå universitet,Klinisk bakteriologi(Swepub:umu)ansj0004 (author)
NRC, KanadaKlinisk bakteriologi (creator_code:org_t)

Related titles

In:Vaccine: Elsevier BV28:7, s. 1824-18310264-410X1873-2518

Internet link

Find in a library

Vaccine (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Conlan, J Wayne; Shen, Hua; Golovliov, Igor, ...; Zingmark, Carl, ...; Oyston, Petra CF; Chen, Wangxue; show more...; House, Robert V; Sjöstedt, Anders ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

Articles in the publication: Vaccine

By the university: Umeå University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se