Search: onr:"swepub:oai:DiVA.org:umu-43179" >
Analyzing p-bodies ...
Analyzing p-bodies and stress granules in Saccharomyces cerevisae
-
Buchan, J Ross (author)
-
- Nissan, Tracy (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
-
Parker, Roy (author)
-
(creator_code:org_t)
- 2
- San Diego : Academic Press, 2010
- 2010
- English.
-
In: Guide to yeast genetics. - San Diego : Academic Press. - 9780123751713 - 9780123751720 ; , s. 619-640
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Eukaryotic cells contain at least two types of cytoplasmic RNA protein (RNP) granules that contain nontranslating mRNAs. One such RNP granule is a P-body, which contains translationally inactive mRNAs and proteins involved in mRNA degradation and translation repression. A second such RNP granule is a stress granule which also contains mRNAs, some RNA binding proteins and several translation initiation factors, suggesting these granules contain mRNAs stalled in translation initiation. In this chapter, we describe methods to analyze P-bodies and stress granules in Saccharomyces cerevisiae, including procedures to determine if a protein or mRNA can accumulate in either granule, if an environmental perturbation or mutation affects granule size and number, and granule quantification methods.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- cytoplasmic processing bodies
- messenger-rna decay
- translational repression
- human-cells
- gene-expression
- global analysis
- body formation
- budding yeast
- localization
- stability
Publication and Content Type
- ref (subject category)
- kap (subject category)
Find in a library
To the university's database