Search: onr:"swepub:oai:DiVA.org:umu-43208" >
CRISPR RNA maturati...
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Deltcheva, ElitzaUmeå universitet,Umeå Centre for Microbial Research (UCMR),Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten)
(author)
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
- Article/chapterEnglish2011
Publisher, publication year, extent ...
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2011-03-30
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Springer Science and Business Media LLC,2011
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-43208
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-43208URI
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https://doi.org/10.1038/nature09886DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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CRISPR/Cas systems constitute a widespread class of immunity systems that protect bacteria and archaea against phages and plasmids, and commonly use repeat/spacer-derived short crRNAs to silence foreign nucleic acids in a sequence-specific manner. Although the maturation of crRNAs represents a key event in CRISPR activation, the responsible endoribonucleases (CasE, Cas6, Csy4) are missing in many CRISPR/Cas subtypes. Here, differential RNA sequencing of the human pathogen Streptococcus pyogenes uncovered tracrRNA, a trans-encoded small RNA with 24-nucleotide complementarity to the repeat regions of crRNA precursor transcripts. We show that tracrRNA directs the maturation of crRNAs by the activities of the widely conserved endogenous RNase III and the CRISPR-associated Csn1 protein; all these components are essential to protect S. pyogenes against prophage-derived DNA. Our study reveals a novel pathway of small guide RNA maturation and the first example of a host factor (RNase III) required for bacterial RNA-mediated immunity against invaders.
Added entries (persons, corporate bodies, meetings, titles ...)
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Chylinski, KrzysztofUmeå universitet,Umeå Centre for Microbial Research (UCMR),Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten)
(author)
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Sharma, Cynthia M
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Gonzales, Karine
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Chao, Yanjie
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Pirzada, Zaid A
(author)
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Eckert, Maria R
(author)
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Vogel, Jörg
(author)
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Charpentier, EmmanuelleUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR)(Swepub:umu)emch0006
(author)
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Umeå universitetUmeå Centre for Microbial Research (UCMR)
(creator_code:org_t)
Related titles
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In:Nature: Springer Science and Business Media LLC471:7340, s. 602-6070028-08361476-4687
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