Search: onr:"swepub:oai:DiVA.org:umu-44220" >
Neuroprotective and...
-
Novikova, Liudmila NUmeå universitet,Anatomi
(author)
Neuroprotective and growth-promoting effects of bone marrow stromal cells after cervical spinal cord injury in adult rats
- Article/chapterEnglish2011
Publisher, publication year, extent ...
-
Elsevier BV,2011
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:umu-44220
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-44220URI
-
https://doi.org/10.3109/14653249.2011.574116DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Background aims. Bone marrow stromal cells (BMSC) have been shown to provide neuroprotection after transplantation into the injured central nervous system. The present study investigated whether adult rat BMSC differentiated along a Schwann cell lineage could increase production of trophic factors and support neuronal survival and axonal regeneration after transplantation into the injured spinal cord. Methods. After cervical C4 hemi-section, 5-bromo-2-deoxyuridine (BrdU)/green fluorescent protein (GFP)-labeled BMSC were injected into the lateral funiculus at 1 mm rostral and caudal to the lesion site. Spinal cords were analyzed 2-13 weeks after transplantation. Results and Conclusions. Treatment of native BMSC with Schwann cell-differentiating factors significantly increased production of brain-derived neurotrophic factor in vitro. Transplanted undifferentiated and differentiated BMSC remained at the injection sites, and in the trauma zone were often associated with neurofilament-positive fibers and increased levels of vascular endothelial growth factor. BMSC promoted extensive in-growth of serotonin-positive raphaespinal axons and calcitonin gene-related peptide (CGRP)-positive dorsal root sensory axons into the trauma zone, and significantly attenuated astroglial and microglial cell reactions, but induced aberrant sprouting of CGRP-immunoreactive axons in Rexed's lamina III. Differentiated BMSC provided neuroprotection for axotomized rubrospinal neurons and increased the density of rubrospinal axons in the dorsolateral funiculus rostral to the injury site. The present results suggest that BMSC induced along the Schwann cell lineage increase expression of trophic factors and have neuroprotective and growth-promoting effects after spinal cord injury.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Brohlin, MariaUmeå universitet,Anatomi(Swepub:umu)mabr0001
(author)
-
Kingham, Paul JUmeå universitet,Anatomi(Swepub:umu)paki0006
(author)
-
Novikov, Lev NUmeå universitet,Anatomi(Swepub:umu)leno0001
(author)
-
Wiberg, MikaelUmeå universitet,Anatomi,Handkirurgi(Swepub:umu)miwi0004
(author)
-
Umeå universitetAnatomi
(creator_code:org_t)
Related titles
-
In:Cytotherapy: Elsevier BV13:7, s. 873-8871465-32491477-2566
Internet link
Find in a library
To the university's database