Search: onr:"swepub:oai:DiVA.org:umu-44748" >
Molecular cloning a...
Molecular cloning and expression of the large subunit of ADP-glucose pyrophosphorylase from barley (Hordeum vulgare) leaves
-
Eimert, K (author)
-
Luo, C (author)
-
Dejardin, A (author)
-
show more...
-
Villand, P (author)
-
Thorbjornsen, T (author)
-
- Kleczkowski, Leszek A, 1954- (author)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Leszek Kleczkowski
-
show less...
-
(creator_code:org_t)
- 1997
- 1997
- English.
-
In: Gene. - 0378-1119 .- 1879-0038. ; 189:1, s. 79-82
- Related links:
-
https://urn.kb.se/re...
Abstract
Subject headings
Close
- A cDNA clone, blpl14, corresponding to the large subunit of ADP-glucose pyrophosphorylase (AGPase), has been isolated from a cDNA library prepared from leaves of barley (Hordeum vulgare L.). An open reading frame encodes a protein of 503 aa, with a calculated molecular weight of 54 815. The derived aa sequence contains a putative transit peptide sequence, required for targeting to plastids, and has a highly conserved positioning of critical Lys residues that are believed to be involved in effector binding. The derived aa sequence shows 97% identity with the corresponding protein from wheat, but only 36% identity with AGPase from E. coli. The blpl14 gene is expressed predominantly in leaves and to a lesser degree in seed endosperm, but not roots, of barley.
Keyword
- chloroplast
- endosperm
- starch
- sucrose
- transit peptide
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Gene
(Search for host publication in LIBRIS)
To the university's database