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Cloning and sequencing of human kappa-casein cDNA.
- Bergström, S (author)
- Hansson, L (author)
-
- Hernell, Olle (author)
- Umeå universitet,Pediatrik
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- Lönnerdal, B (author)
- Nilsson, A K (author)
- Strömqvist, M (author)
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- (creator_code:org_t)
- 1992
- 1992
- English.
- In: DNA Sequence. - 1042-5179 .- 1029-2365. ; 3:4, s. 245-6
- Journal article (peer-reviewed)
Abstract
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- A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.
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- Bergström, S
- Hansson, L
- Hernell, Olle
- Lönnerdal, B
- Nilsson, A K
- Strömqvist, M
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- DNA Sequence
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- Umeå University
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