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Cofilin regulator 1...
Cofilin regulator 14-3-3zeta is an evolutionarily conserved protein required for phagocytosis and microbial resistance
- Article/chapterEnglish2011
Publisher, publication year, extent ...
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2011-01-04
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Society for Leukocyte Biology,2011
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-46033
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-46033URI
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https://doi.org/10.1189/jlb.0410195DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3ζ, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3ζ had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3ζ was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3ζ was required for resistance against Staphylococcus aureus. We conclude that 14-3-3ζ is essential for phagocytosis and microbial resistance in insects and vertebrates.
Subject headings and genre
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Drosophila
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zebrafish
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plasmatocyte
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macrophage
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immunity
Added entries (persons, corporate bodies, meetings, titles ...)
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Vanha-aho, Leena-Maija
(author)
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Kleino, AnniUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
(author)
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Vähä-Mäkilä, Mari
(author)
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Vuoksio, Milka
(author)
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Eskelinen, Sinikka
(author)
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Hultmark, DanUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Hultmark(Swepub:umu)dahu0001
(author)
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Kocks, Christine
(author)
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Hallman, Mikko
(author)
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Parikka, Mataleena
(author)
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Rämet, Mika
(author)
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Umeå universitetInstitutionen för molekylärbiologi (Medicinska fakulteten)
(creator_code:org_t)
Related titles
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In:Journal of Leukocyte Biology: Society for Leukocyte Biology89:5, s. 649-6590741-54001938-3673
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Ulvila, Johanna
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Vanha-aho, Leena ...
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Kleino, Anni
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Vähä-Mäkilä, Mar ...
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Vuoksio, Milka
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Eskelinen, Sinik ...
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Hultmark, Dan
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Kocks, Christine
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Hallman, Mikko
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Parikka, Matalee ...
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Rämet, Mika
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Journal of Leuko ...
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Umeå University