SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:umu-46223"
 

Search: onr:"swepub:oai:DiVA.org:umu-46223" > Plasticity in patte...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Riddle, Nicole CDepartment of Biology, Washington University St. Louis, Missouri 63130, USA (author)

Plasticity in patterns of histone modifications and chromosomal proteins in Drosophila heterochromatin

  • Article/chapterEnglish2011

Publisher, publication year, extent ...

  • 2010-12-22
  • Cold Spring Harbor Laboratory,2011
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:umu-46223
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-46223URI
  • https://doi.org/10.1101/gr.110098.110DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Eukaryotic genomes are packaged in two basic forms, euchromatin and heterochromatin. We have examined the composition and organization of Drosophila melanogaster heterochromatin in different cell types using ChIP-array analysis of histone modifications and chromosomal proteins. As anticipated, the pericentric heterochromatin and chromosome 4 are on average enriched for the "silencing" marks H3K9me2, H3K9me3, HP1a, and SU(VAR)3-9, and are generally depleted for marks associated with active transcription. The locations of the euchromatin-heterochromatin borders identified by these marks are similar in animal tissues and most cell lines, although the amount of heterochromatin is variable in some cell lines. Combinatorial analysis of chromatin patterns reveals distinct profiles for euchromatin, pericentric heterochromatin, and the 4th chromosome. Both silent and active protein-coding genes in heterochromatin display complex patterns of chromosomal proteins and histone modifications; a majority of the active genes exhibit both "activation" marks (e.g., H3K4me3 and H3K36me3) and "silencing" marks (e.g., H3K9me2 and HP1a). The hallmark of active genes in heterochromatic domains appears to be a loss of H3K9 methylation at the transcription start site. We also observe complex epigenomic profiles of intergenic regions, repeated transposable element (TE) sequences, and genes in the heterochromatic extensions. An unexpectedly large fraction of sequences in the euchromatic chromosome arms exhibits a heterochromatic chromatin signature, which differs in size, position, and impact on gene expression among cell types. We conclude that patterns of heterochromatin/euchromatin packaging show greater complexity and plasticity than anticipated. This comprehensive analysis provides a foundation for future studies of gene activity and chromosomal functions that are influenced by or dependent upon heterochromatin.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Minoda, AkiDepartment of Molecular and Cell Biology, University of California at Berkeley and Department of Genome Dynamics, Lawrence Berkeley National Lab, Berkeley, California 94720, USA (author)
  • Kharchenko, Peter VCenter for Biomedical Informatics, Harvard Medical School and Informatics Program, Children's Hospital, Boston, Massachusetts 02115, USA (author)
  • Alekseyenko, Artyom ADivision of Genetics, Department of Medicine, Brigham & Women's Hospital, and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA (author)
  • Schwartz, Yuri BUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Molecular Biology & Biochemistry, Rutgers University, Piscataway, New Jersey 08901, USA(Swepub:umu)yusc0002 (author)
  • Tolstorukov, Michael YCenter for Biomedical Informatics, Harvard Medical School and Informatics Program, Children's Hospital, Boston, Massachusetts 02115, USA (author)
  • Gorchakov, Andrey ADivision of Genetics, Department of Medicine, Brigham & Women's Hospital, and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA (author)
  • Jaffe, Jacob DProteomics Group, The Broad Institute, Cambridge, Massachusetts 02139, USA (author)
  • Kennedy, CameronDepartment of Molecular and Cell Biology, University of California at Berkeley and Department of Genome Dynamics, Lawrence Berkeley National Lab, Berkeley, California 94720, USA (author)
  • Linder-Basso, DanielaDepartment of Molecular Biology & Biochemistry, Rutgers University, Piscataway, New Jersey 08901, USA (author)
  • Peach, Sally EProteomics Group, The Broad Institute, Cambridge, Massachusetts 02139, USA (author)
  • Shanower, GregoryDepartment of Molecular Biology & Biochemistry, Rutgers University, Piscataway, New Jersey 08901, USA (author)
  • Zheng, HaiyanBiological Mass Spectrometry Resource, Center for Advanced Biotechnology and Medicine, University of Dentistry and Medicine of New Jersey, Piscataway, New Jersey 08854, USA (author)
  • Kuroda, Mitzi IDivision of Genetics, Department of Medicine, Brigham & Women's Hospital, and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA (author)
  • Pirrotta, VincenzoDepartment of Molecular Biology & Biochemistry, Rutgers University, Piscataway, New Jersey 08901, USA (author)
  • Park, Peter JCenter for Biomedical Informatics, Harvard Medical School and Informatics Program, Children's Hospital, Boston, Massachusetts 02115, USA (author)
  • Elgin, Sarah C RDepartment of Biology, Washington University St. Louis, Missouri 63130, USA (author)
  • Karpen, Gary HDepartment of Molecular and Cell Biology, University of California at Berkeley and Department of Genome Dynamics, Lawrence Berkeley National Lab, Berkeley, California 94720, USA (author)
  • Department of Biology, Washington University St. Louis, Missouri 63130, USADepartment of Molecular and Cell Biology, University of California at Berkeley and Department of Genome Dynamics, Lawrence Berkeley National Lab, Berkeley, California 94720, USA (creator_code:org_t)

Related titles

  • In:Genome Research: Cold Spring Harbor Laboratory21:2, s. 147-1631088-90511549-5469

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view