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Analyzing P-bodies in Saccharomyces cerevisiae
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- Nissan, Tracy (author)
- The University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA
- Parker, Roy (author)
- (creator_code:org_t)
- 2008
- 2008
- English.
- In: Methods in Enzymology. - 0076-6879 .- 1557-7988. ; 448, s. 507-520
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Cytoplasmic processing bodies, or P-bodies, are RNA-protein granules found in eukaryotic cells. P-bodies contain non-translating mRNAs and proteins involved in mRNA degradation and translational repression. P-bodies, and the mRNPs within them, have been implicated in mRNA storage, mRNA degradation, and translational repression. The analysis of mRNA turnover often involves the analysis of P-bodies. In this chapter, we describe methods to analyze P-bodies in the budding yeast, Saccharomyces cerevisiae, including procedures to determine whether a protein or mRNA can accumulate in P-bodies, whether an environmental perturbation or mutation affects P-body size and number, and methods to quantify P-bodies.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- cytoplasmic processing bodies messenger-rna decay translational repression human-cells gene-expression global analysis body formation budding yeast localization stability
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