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Analyzing P-bodies ...
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Nissan, TracyThe University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA
(author)
Analyzing P-bodies in Saccharomyces cerevisiae
- Article/chapterEnglish2008
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LIBRIS-ID:oai:DiVA.org:umu-46251
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-46251URI
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https://doi.org/10.1016/S0076-6879(08)02625-6DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Cytoplasmic processing bodies, or P-bodies, are RNA-protein granules found in eukaryotic cells. P-bodies contain non-translating mRNAs and proteins involved in mRNA degradation and translational repression. P-bodies, and the mRNPs within them, have been implicated in mRNA storage, mRNA degradation, and translational repression. The analysis of mRNA turnover often involves the analysis of P-bodies. In this chapter, we describe methods to analyze P-bodies in the budding yeast, Saccharomyces cerevisiae, including procedures to determine whether a protein or mRNA can accumulate in P-bodies, whether an environmental perturbation or mutation affects P-body size and number, and methods to quantify P-bodies.
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Parker, Roy
(author)
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The University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA
(creator_code:org_t)
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In:Methods in Enzymology448, s. 507-5200076-68791557-7988
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