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  • Nissan, TracyThe University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA (author)

Analyzing P-bodies in Saccharomyces cerevisiae

  • Article/chapterEnglish2008

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  • 2008
  • printrdacarrier

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  • LIBRIS-ID:oai:DiVA.org:umu-46251
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-46251URI
  • https://doi.org/10.1016/S0076-6879(08)02625-6DOI

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  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype

Notes

  • Cytoplasmic processing bodies, or P-bodies, are RNA-protein granules found in eukaryotic cells. P-bodies contain non-translating mRNAs and proteins involved in mRNA degradation and translational repression. P-bodies, and the mRNPs within them, have been implicated in mRNA storage, mRNA degradation, and translational repression. The analysis of mRNA turnover often involves the analysis of P-bodies. In this chapter, we describe methods to analyze P-bodies in the budding yeast, Saccharomyces cerevisiae, including procedures to determine whether a protein or mRNA can accumulate in P-bodies, whether an environmental perturbation or mutation affects P-body size and number, and methods to quantify P-bodies.

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  • Parker, Roy (author)
  • The University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA (creator_code:org_t)

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  • In:Methods in Enzymology448, s. 507-5200076-68791557-7988

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Umeå University

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