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Aging effect on neu...
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Brohlin, MariaUmeå universitet,Anatomi,Neurogruppen
(author)
Aging effect on neurotrophic activity of human mesenchymal stem cells
- Article/chapterEnglish2012
Publisher, publication year, extent ...
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2012-09-17
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San Francisco :Public Library of Science,2012
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-47755
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-47755URI
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https://doi.org/10.1371/journal.pone.0045052DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Clinical efficacy of stem cells for nerve repair is likely to be influenced by issues including donor age and in vitro expansion time. We isolated human mesenchymal stem cells (MSC) from bone marrow of young (16–18 years) and old (67–75 years) donors and analyzed their capacity to differentiate and promote neurite outgrowth from dorsal root ganglia (DRG) neurons. Treatment of MSC with growth factors (forskolin, basic fibroblast growth factor, platelet derived growth factor-AA and glial growth factor-2) induced protein expression of the glial cell marker S100 in cultures from young but not old donors. MSC expressed various neurotrophic factor mRNA transcripts. Growth factor treatment enhanced the levels of BDNF and VEGF transcripts with corresponding increases in protein release in both donor cell groups. MSC in co-culture with DRG neurons significantly enhanced total neurite length which, in the case of young but not old donors, was further potentiated by treatment of the MSC with the growth factors. Stem cells from young donors maintained their proliferation rate over a time course of 9 weeks whereas those from the old donors showed increased population doubling times. MSC from young donors, differentiated with growth factors after long-term culture, maintained their ability to enhance neurite outgrowth of DRG. Therefore, MSC isolated from young donors are likely to be a favourable cell source for nerve repair.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Kingham, PaulUmeå universitet,Anatomi,Neurogruppen(Swepub:umu)paki0006
(author)
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Novikova, LiudmilaUmeå universitet,Anatomi,Neurogruppen(Swepub:umu)luno0001
(author)
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Novikov, LevUmeå universitet,Anatomi,Neurogruppen(Swepub:umu)leno0001
(author)
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Wiberg, MikaelUmeå universitet,Anatomi,Handkirurgi(Swepub:umu)miwi0004
(author)
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Umeå universitetAnatomi
(creator_code:org_t)
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In:PLOS ONESan Francisco : Public Library of Science7:9, s. e45052-1932-6203
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