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ND3 Cys39 in comple...
ND3 Cys39 in complex I is exposed mitochondrial
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Burger, N. (author)
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James, A. M. (author)
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Mulvey, J. F. (author)
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Hoogewijs, K. (author)
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Ding, S. J. (author)
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Fearnley, I. M. (author)
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Loureiro-Lopez, M. (author)
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Norman, A. A. I. (author)
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Arndt, S. (author)
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- Mottahedin, Amin (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology
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Sauchanka, O. (author)
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Hartley, R. C. (author)
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Krieg, T. (author)
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Murphy, M. P. (author)
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
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In: Cell Chemical Biology. - : Elsevier BV. - 2451-9448 .- 2451-9456. ; 29:4
- Related links:
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http://www.cell.com/...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Mammalian complex I can adopt catalytically active (A-) or deactive (D-) states. A defining feature of the reversible transition between these two defined states is thought to be exposure of the ND3 subunit Cys39 residue in the D-state and its occlusion in the A-state. As the catalytic A/D transition is important in health and disease, we set out to quantify it by measuring Cys39 exposure using isotopic labeling and mass spectrometry, in parallel with complex I NADH/CoQ oxidoreductase activity. To our surprise, we found significant Cys39 exposure during NADH/CoQ oxidoreductase activity. Furthermore, this activity was unaffected if Cys39 alkylation occurred during complex I-linked respiration. In contrast, alkylation of catalytically inactive complex I irreversibly blocked the reactivation of NADH/CoQ oxidoreductase activity by NADH. Thus, Cys39 of ND3 is exposed in complex I during mitochondrial respiration, with significant implications for our understanding of the A/D transition and the mechanism of complex I.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences (hsv//eng)
Keyword
- active/de-active transition
- s-nitrosation
- superoxide-production
- catalytic-properties
- nadh
- state
- protein
- membrane
- translocation
- respiration
- Biochemistry & Molecular Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Burger, N.
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James, A. M.
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Mulvey, J. F.
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Hoogewijs, K.
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Ding, S. J.
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Fearnley, I. M.
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show more...
-
Loureiro-Lopez, ...
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Norman, A. A. I.
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Arndt, S.
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Mottahedin, Amin
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Sauchanka, O.
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Hartley, R. C.
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Krieg, T.
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Murphy, M. P.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Health Sciences
- Articles in the publication
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Cell Chemical Bi ...
- By the university
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University of Gothenburg