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ND3 Cys39 in complex I is exposed mitochondrial
- Burger, N. (author)
- James, A. M. (author)
- Mulvey, J. F. (author)
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- Hoogewijs, K. (author)
- Ding, S. J. (author)
- Fearnley, I. M. (author)
- Loureiro-Lopez, M. (author)
- Norman, A. A. I. (author)
- Arndt, S. (author)
- Sauchanka, O. (author)
- Hartley, R. C. (author)
- Krieg, T. (author)
- Murphy, M. P. (author)
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- (creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
- In: Cell Chemical Biology. - : Elsevier BV. - 2451-9448 .- 2451-9456. ; 29:4
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Mammalian complex I can adopt catalytically active (A-) or deactive (D-) states. A defining feature of the reversible transition between these two defined states is thought to be exposure of the ND3 subunit Cys39 residue in the D-state and its occlusion in the A-state. As the catalytic A/D transition is important in health and disease, we set out to quantify it by measuring Cys39 exposure using isotopic labeling and mass spectrometry, in parallel with complex I NADH/CoQ oxidoreductase activity. To our surprise, we found significant Cys39 exposure during NADH/CoQ oxidoreductase activity. Furthermore, this activity was unaffected if Cys39 alkylation occurred during complex I-linked respiration. In contrast, alkylation of catalytically inactive complex I irreversibly blocked the reactivation of NADH/CoQ oxidoreductase activity by NADH. Thus, Cys39 of ND3 is exposed in complex I during mitochondrial respiration, with significant implications for our understanding of the A/D transition and the mechanism of complex I.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences (hsv//eng)
Keyword
- active/de-active transition
- s-nitrosation
- superoxide-production
- catalytic-properties
- nadh
- state
- protein
- membrane
- translocation
- respiration
- Biochemistry & Molecular Biology
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- ref (subject category)
- art (subject category)
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- Burger, N.
- James, A. M.
- Mulvey, J. F.
- Hoogewijs, K.
- Ding, S. J.
- Fearnley, I. M.
- show more...
- Loureiro-Lopez, ...
- Norman, A. A. I.
- Arndt, S.
- Mottahedin, Amin
- Sauchanka, O.
- Hartley, R. C.
- Krieg, T.
- Murphy, M. P.
- show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
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- University of Gothenburg
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