Search: onr:"swepub:oai:DiVA.org:umu-4945" >
Rational DOE-protoc...
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Olsson, Ing-MarieUmeå universitet,Kemiska institutionen
(author)
Rational DOE-protocols for 96-well plates
- Article/chapterEnglish2006
Publisher, publication year, extent ...
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Amsterdam :Elsevier,2006
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-4945
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-4945URI
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https://doi.org/10.1016/j.chemolab.2006.01.005DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The use of 96-well plates for chemical and biological applications has rapidly increased as new applicable domains have been discovered and new laboratory instruments developed. There are 96, 384, 1536, etc. plates customized for diverse applications such as biological assays, sample preparation, solid-phase extraction and crystallization. Multi-pipettes as well as automated pipette systems accelerate the preparation of plates resulting in even faster evaluation systems. A bottleneck in the use of multi-unit plates is method development and optimization. By applying rational experimental design, the optimization could be made more efficient and less time-consuming. Unfortunately, the workload related to manual preparation of multi-unit plates according to an experimental design is often considered overwhelming. The present study introduces a new approach for experimental design in 96-well plates that minimizes the manual workload without compromising the quality of the experimental design. This approach is scalable to larger rectangular formats such as 384- and 1536-well plates. The optimal combinations will be delineated and applied experimentally to a reporter-gene assay.
Subject headings and genre
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DOE
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96-well technology
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RED-MUP
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Assay optimisation
Added entries (persons, corporate bodies, meetings, titles ...)
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Johansson, ErikUmeå universitet,Kemiska institutionen
(author)
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Berntsson, Martin
(author)
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Eriksson, Lennart
(author)
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Gottfries, Johan
(author)
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Wold, SvanteUmeå universitet,Kemiska institutionen,Research Group for Chemometrics(Swepub:umu)svwo0001
(author)
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Umeå universitetKemiska institutionen
(creator_code:org_t)
Related titles
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In:Chemometrics and Intelligent Laboratory SystemsAmsterdam : Elsevier83:1, s. 66-740169-74391873-3239
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