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The endocytic prote...
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Doherty, Gary J.
(author)
The endocytic protein GRAF1 is directed to cell-matrix adhesion sites and regulates cell spreading
- Article/chapterEnglish2011
Publisher, publication year, extent ...
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Bethesda :American Society for Cell Biology,2011
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-50518
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-50518URI
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https://doi.org/10.1091/mbc.E10-12-0936DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The rho GTPase-activating protein GTPase regulator associated with focal adhesion kinase-1 (GRAF1) remodels membranes into tubulovesicular clathrin-independent carriers (CLICs) mediating lipid-anchored receptor endocytosis. However, the cell biological functions of this highly prevalent endocytic pathway are unclear. In this article, we present biochemical and cell biological evidence that GRAF1 interacted with a network of endocytic and adhesion proteins and was found enriched at podosome-like adhesions and src-induced podosomes. We further demonstrate that these sites comprise microdomains of highly ordered lipid enriched in GRAF1 endocytic cargo. GRAF1 activity was upregulated in spreading cells and uptake via CLICs was concentrated at the leading edge of migrating cells. Depletion of GRAF1, which inhibits CLIC generation, resulted in profound defects in cell spreading and migration. We propose that GRAF1 remodels membrane microdomains at adhesion sites into endocytic carriers, facilitating membrane turnover during cell morphological changes.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Åhlund, Monika K.Umeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)moaahd01
(author)
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Howes, Mark T.
(author)
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Moren, Bjorn
(author)
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Parton, Robert G.
(author)
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McMahon, Harvey T.
(author)
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Lundmark, RichardUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)rilu0004
(author)
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Umeå universitetInstitutionen för medicinsk kemi och biofysik
(creator_code:org_t)
Related titles
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In:Molecular Biology of the CellBethesda : American Society for Cell Biology22:22, s. 4380-43891059-15241939-4586
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