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Caenorhabditis elegans Numb Inhibits Endocytic Recycling by Binding TAT-1 Aminophospholipid Translocase
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- Nilsson, Lars (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Simon Tuck
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- Jonsson, Eva (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Simon Tuck
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- Tuck, Simon (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Simon Tuck
- (creator_code:org_t)
- 2011-09-14
- 2011
- English.
- In: Traffic. - Malden : Wiley-Blackwell. - 1398-9219 .- 1600-0854. ; 12:12, s. 1839-1849
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Numb regulates endocytosis in many metazoans, but the mechanism by which it functions is not completely understood. Here we report that the Caenorhabditis ele-gans Numb ortholog, NUM-1A, a regulator of endocytic recycling, binds the C isoform of transbilayer amphipath transporter-1 (TAT-1), a P4 family adenosine triphosphatase and putative aminophospholipid translocase that is required for proper endocytic trafficking. We demonstrate that TAT-1 is differentially spliced during development and that TAT-1C-specific splicing occurs in the intestine where NUM-1A is known to function. NUM-1A and TAT-1C colocalize in vivo. We have mapped the binding site to an NXXF motif in TAT-1C. This motif is not required for TAT-1C function but is required for NUM-1A's ability to inhibit recycling. We demonstrate that num-1A and tat-1 defects are both suppressed by the loss of the activity of PSSY-1, a phosphatidylserine (PS) synthase. PS is mislocalized in intestinal cells with defects in tat-1 or num-1A function. We propose that NUM-1A inhibits recycling by inhibiting TAT-1C's ability to translocate PS across the membranes of recycling endosomes.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- C. elegans
- endocytosis
- numb
- phosphatidylserine
- recycling
- tat-1
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- ref (subject category)
- art (subject category)
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