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LcrV is a channel s...
LcrV is a channel size-determining component of the Yop effector translocon of Yersinia
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- Holmström, Anna (author)
- Swedish Defence Research Agency, Division of CBRN Defence and Security, SE-901 82 Umeå, Sweden
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- Olsson, Jan (author)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)
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Cherepanov, Peter (author)
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Maier, Elke (author)
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- Nordfelth, Roland (author)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)
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- Pettersson, Jonas, 1979- (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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Benz, Roland (author)
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- Wolf-Watz, Hans (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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Forsberg, Åke, 1951- (author)
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(creator_code:org_t)
- Blackwell Publishing, 2001
- 2001
- English.
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In: Molecular Microbiology. - : Blackwell Publishing. - 0950-382X .- 1365-2958. ; 39:3, s. 620-632
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Abstract
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- Delivery of Yop effector proteins by pathogenic Yersinia across the eukaryotic cell membrane requires LcrV, YopB and YopD. These proteins were also required for channel formation in infected erythrocytes and, using different osmolytes, the contact‐dependent haemolysis assay was used to study channel size. Channels associated with LcrV were around 3 nm, whereas the homologous PcrV protein of Pseudomonas aeruginosa induced channels of around 2 nm in diameter. In lipid bilayer membranes, purified LcrV and PcrV induced a stepwise conductance increase of 3 nS and 1 nS, respectively, in 1 M KCl. The regions important for channel size were localized to amino acids 127–195 of LcrV and to amino acids 106–173 of PcrV. The size of the channel correlated with the ability to translocate Yop effectors into host cells. We suggest that LcrV is a size‐determining structural component of the Yop translocon.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Holmström, Anna
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Olsson, Jan
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Cherepanov, Pete ...
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Maier, Elke
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Nordfelth, Rolan ...
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Pettersson, Jona ...
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show more...
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Benz, Roland
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Wolf-Watz, Hans
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Forsberg, Åke, 1 ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Molecular Microb ...
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Umeå University