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Francisella is sens...
Francisella is sensitive to insect antimicrobial peptides
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- Vonkavaara, Malin, 1981- (author)
- Umeå universitet,Klinisk bakteriologi
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- Pavel, Shaikh Terkis Islam (author)
- Umeå universitet,Klinisk bakteriologi
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- Hölzl, Kathrin (author)
- Umeå universitet,Klinisk bakteriologi
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- Nordfelth, Roland (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Sjöstedt, Anders (author)
- Umeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS),Arcum
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- Stöven, Svenja (author)
- Umeå universitet,Europeiska CBRNE-centret
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(creator_code:org_t)
- 2012-09-29
- 2013
- English.
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In: Journal of Innate Immunity. - Basel : Karger. - 1662-811X .- 1662-8128. ; 5:1, s. 50-59
- Related links:
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https://www.karger.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Francisella tularensis causes the zoonotic disease tularemia. Arthropod vectors are important transmission routes for the disease, although it is not known how Francisella survives the efficient arthropod immune response. Here, we used Drosophila melanogaster as a model host for Francisella infections and investigated whether the bacteria are resistant to insect humoral immune responses, in particular to the antimicrobial peptides (AMPs) secreted into the insect hemolymph. Moreover, we asked to which extent such resistance might depend on LPS structure and surface characteristics of the bacteria. We analyzed F. novicida mutant strains in genes, directly or indirectly involved in specific steps of LPS biosynthesis, for virulence in wildtype and Relish E20 immune deficient flies, and tested selected mutants for sensitivity to AMPs in vitro. We demonstrate that Francisella is sensitive to specific fly AMPs, i.e. Attacin, Cecropin, Drosocin and Drosomycin. Furthermore, six bacterial genes, kpsF, manB, lpxF, slt, tolA and pal, were found to be required for resistance to Relish-dependent immune responses, illustrating the importance of structural details of Francisella lipid A and Kdo core for interactions with AMPs. Interestingly, a more negative surface charge and lack of O-antigen did not render mutant bacteria more sensitive to cationic AMPs and attenuated virulence in flies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Francisella tularensis
- Drosophila melanogaster
- Antimicrobial peptides
- Lipopolysaccharide
- Host-pathogen interactions
- Clinical Bacteriology
- klinisk bakteriologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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