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Search: onr:"swepub:oai:DiVA.org:umu-55680" > Sequence-Specific T...

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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004297naa a2200529 4500
001oai:DiVA.org:umu-55680
003SwePub
008120528s2012 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-556802 URI
0247 a https://doi.org/10.1371/journal.pgen.10026462 DOI
040 a (SwePub)umu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Alekeyenko, Artyom A.4 aut
2451 0a Sequence-Specific Targeting of Dosage Compensation in Drosophila Favors an Active Chromatin Context
264 c 2012-04-26
264 1a San Francisco :b Public Library of Science,c 2012
338 a electronic2 rdacarrier
520 a The Drosophila MSL complex mediates dosage compensation by increasing transcription of the single X chromosome in males approximately two-fold. This is accomplished through recognition of the X chromosome and subsequent acetylation of histone H4K16 on X-linked genes. Initial binding to the X is thought to occur at "entry sites" that contain a consensus sequence motif ("MSL recognition element" or MRE). However, this motif is only similar to 2 fold enriched on X, and only a fraction of the motifs on X are initially targeted. Here we ask whether chromatin context could distinguish between utilized and non-utilized copies of the motif, by comparing their relative enrichment for histone modifications and chromosomal proteins mapped in the modENCODE project. Through a comparative analysis of the chromatin features in male S2 cells (which contain MSL complex) and female Kc cells (which lack the complex), we find that the presence of active chromatin modifications, together with an elevated local GC content in the surrounding sequences, has strong predictive value for functional MSL entry sites, independent of MSL binding. We tested these sites for function in Kc cells by RNAi knockdown of Sxl, resulting in induction of MSL complex. We show that ectopic MSL expression in Kc cells leads to H4K16 acetylation around these sites and a relative increase in X chromosome transcription. Collectively, our results support a model in which a pre-existing active chromatin environment, coincident with H3K36me3, contributes to MSL entry site selection. The consequences of MSL targeting of the male X chromosome include increase in nucleosome lability, enrichment for H4K16 acetylation and JIL-1 kinase, and depletion of linker histone H1 on active X-linked genes. Our analysis can serve as a model for identifying chromatin and local sequence features that may contribute to selection of functional protein binding sites in the genome.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
7001 a Ho, Joshua W. K.4 aut
7001 a Peng, Shouyong4 aut
7001 a Gelbart, Marnie4 aut
7001 a Tolstorukov, Michael Y.4 aut
7001 a Plachetka, Annette4 aut
7001 a Kharchenko, Peter V.4 aut
7001 a Jung, Youngsook L.4 aut
7001 a Gorchakov, Andrey A.4 aut
7001 a Larschan, Erica4 aut
7001 a Gu, Tingting4 aut
7001 a Minoda, Aki4 aut
7001 a Riddle, Nicole C.4 aut
7001 a Schwartz, Yuri B.u Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Schwartz4 aut0 (Swepub:umu)yusc0002
7001 a Elgin, Sarah C. R.4 aut
7001 a Karpen, Gary H.4 aut
7001 a Pirrotta, Vincenzo4 aut
7001 a Kuroda, Mitzi I.4 aut
7001 a Park, Peter J.4 aut
7102 a Umeå universitetb Institutionen för molekylärbiologi (Medicinska fakulteten)4 org
7730 t PLoS Geneticsd San Francisco : Public Library of Scienceg 8:4, s. e1002646-q 8:4<e1002646-x 1553-7390x 1553-7404
8564 u https://umu.diva-portal.org/smash/get/diva2:528844/FULLTEXT02.pdfx primaryx Raw objecty fulltext:print
8564 u https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1002646&type=printable
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-55680
8564 8u https://doi.org/10.1371/journal.pgen.1002646

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