Search: onr:"swepub:oai:DiVA.org:umu-58142" >
Nature and function...
-
Schwartz, Yuri BUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Schwartz
(author)
Nature and function of insulator protein binding sites in the Drosophila genome
- Article/chapterEnglish2012
Publisher, publication year, extent ...
-
2012-07-05
-
Cold Spring Harbor Laboratory,2012
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:umu-58142
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-58142URI
-
https://doi.org/10.1101/gr.138156.112DOI
Supplementary language notes
-
Language:English
-
Summary in:English &language:-1_t
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Chromatin insulator elements and associated proteins have been proposed to partition eukaryotic genomes into sets of independently regulated domains. Here we test this hypothesis by quantitative genome-wide analysis of insulator protein binding to Drosophila chromatin. We find distinct combinatorial binding of insulator proteins to different classes of sites and uncover a novel type of insulator element that binds CP190 but not any other known insulator proteins. Functional characterization of different classes of binding sites indicates that only a small fraction act as robust insulators in standard enhancer-blocking assays. We show that insulators restrict the spreading of the H3K27me3 mark but only at a small number of Polycomb target regions and only to prevent repressive histone methylation within adjacent genes that are already transcriptionally inactive. RNAi knockdown of insulator proteins in cultured cells does not lead to major alterations in genome expression. Taken together these observations argue against the concept of a genome partitioned by specialized boundary elements and suggest that insulators are reserved for specific regulation of selected genes.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Linder-Basso, Daniela
(author)
-
Kharchenko, Peter V
(author)
-
Tolstorukov, Michael Y
(author)
-
Kim, MariaUmeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Larsson(Swepub:umu)maki0039
(author)
-
Li, Hua-Bing
(author)
-
Gorchakov, Andrey A
(author)
-
Minoda, Aki
(author)
-
Shanower, Gregory
(author)
-
Alekseyenko, Artyom A
(author)
-
Riddle, Nicole C
(author)
-
Jung, Youngsook L
(author)
-
Gu, Tingting
(author)
-
Plachetka, Annette
(author)
-
Elgin, Sarah CR
(author)
-
Kuroda, Mitzi I
(author)
-
Park, Peter J
(author)
-
Savitsky, MikhailUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Schwartz(Swepub:umu)misa0064
(author)
-
Karpen, Gary H
(author)
-
Pirrotta, Vincenzo
(author)
-
Umeå universitetInstitutionen för molekylärbiologi (Medicinska fakulteten)
(creator_code:org_t)
Related titles
-
In:Genome Research: Cold Spring Harbor Laboratory22, s. 2188-21981088-90511549-5469
Internet link
Find in a library
To the university's database
- By the author/editor
-
Schwartz, Yuri B
-
Linder-Basso, Da ...
-
Kharchenko, Pete ...
-
Tolstorukov, Mic ...
-
Kim, Maria
-
Li, Hua-Bing
-
show more...
-
Gorchakov, Andre ...
-
Minoda, Aki
-
Shanower, Gregor ...
-
Alekseyenko, Art ...
-
Riddle, Nicole C
-
Jung, Youngsook ...
-
Gu, Tingting
-
Plachetka, Annet ...
-
Elgin, Sarah CR
-
Kuroda, Mitzi I
-
Park, Peter J
-
Savitsky, Mikhai ...
-
Karpen, Gary H
-
Pirrotta, Vincen ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Microbiology in ...
- Articles in the publication
-
Genome Research
- By the university
-
Umeå University