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  • Schwartz, Yuri BUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Schwartz (author)

Nature and function of insulator protein binding sites in the Drosophila genome

  • Article/chapterEnglish2012

Publisher, publication year, extent ...

  • 2012-07-05
  • Cold Spring Harbor Laboratory,2012
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:umu-58142
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-58142URI
  • https://doi.org/10.1101/gr.138156.112DOI

Supplementary language notes

  • Language:English
  • Summary in:English &language:-1_t

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Chromatin insulator elements and associated proteins have been proposed to partition eukaryotic genomes into sets of independently regulated domains. Here we test this hypothesis by quantitative genome-wide analysis of insulator protein binding to Drosophila chromatin. We find distinct combinatorial binding of insulator proteins to different classes of sites and uncover a novel type of insulator element that binds CP190 but not any other known insulator proteins. Functional characterization of different classes of binding sites indicates that only a small fraction act as robust insulators in standard enhancer-blocking assays. We show that insulators restrict the spreading of the H3K27me3 mark but only at a small number of Polycomb target regions and only to prevent repressive histone methylation within adjacent genes that are already transcriptionally inactive. RNAi knockdown of insulator proteins in cultured cells does not lead to major alterations in genome expression. Taken together these observations argue against the concept of a genome partitioned by specialized boundary elements and suggest that insulators are reserved for specific regulation of selected genes.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Linder-Basso, Daniela (author)
  • Kharchenko, Peter V (author)
  • Tolstorukov, Michael Y (author)
  • Kim, MariaUmeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Larsson(Swepub:umu)maki0039 (author)
  • Li, Hua-Bing (author)
  • Gorchakov, Andrey A (author)
  • Minoda, Aki (author)
  • Shanower, Gregory (author)
  • Alekseyenko, Artyom A (author)
  • Riddle, Nicole C (author)
  • Jung, Youngsook L (author)
  • Gu, Tingting (author)
  • Plachetka, Annette (author)
  • Elgin, Sarah CR (author)
  • Kuroda, Mitzi I (author)
  • Park, Peter J (author)
  • Savitsky, MikhailUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Schwartz(Swepub:umu)misa0064 (author)
  • Karpen, Gary H (author)
  • Pirrotta, Vincenzo (author)
  • Umeå universitetInstitutionen för molekylärbiologi (Medicinska fakulteten) (creator_code:org_t)

Related titles

  • In:Genome Research: Cold Spring Harbor Laboratory22, s. 2188-21981088-90511549-5469

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