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Isolation of cDNA s...
Isolation of cDNA sequences coding for a part of human tissue plasminogen activator.
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Edlund, T (author)
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- Ny, Tor (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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Rånby, M (author)
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Hedén, L O (author)
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Palm, G (author)
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Holmgren, E (author)
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Josephson, S (author)
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(creator_code:org_t)
- 1983
- 1983
- English.
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In: Proceedings of the National Academy of Sciences of the United States of America. - 0027-8424 .- 1091-6490. ; 80:2, s. 349-52
- Related links:
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https://urn.kb.se/re...
Abstract
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- We have isolated a cDNA sequence coding for a part of human tissue plasminogen activator. mRNA coding for tissue plasminogen activator was partially purified, copied into double-stranded cDNA, and cloned into Escherichia coli. Two sets of partially overlapping oligodeoxynucleotide mixtures corresponding to all possible coding sequences for a known portion of the tissue plasminogen activator gene were prepared. One set was used as a probe to screen cDNA containing bacterial clones and both were used as probes in hybridization against purified plasmid DNA. Of 4,200 bacterial clones examined, 1 carried a plasmid that hybridized to both sets of oligonucleotides. This plasmid contained a 370-base-pair cDNA insert, which was shown by nucleotide sequence analysis to code for the cleavage site region in the one-chain form of the human tissue plasminogen activator.
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