Evidence for IFN-beta heterogeneity in a substrain of Namalwa cells.

Borg, H (author)

Nordensson, I (author)

Ny, Tor (author): Umeå universitet,Institutionen för medicinsk kemi och biofysik

Lundgren, E (author)

(creator_code:org_t)

1985
1985
English.
In: Applied Biochemistry and Biotechnology. - 0273-2289 .- 1559-0291. ; 11:2, s. 111-22

Abstract Subject headings

A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale, and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-alpha and IFN-beta. More than 80% of the IFN activity was IFN-beta by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 10(6) U/mg protein. The IFN-beta type was found to be heterogeneous, and could be separated into several components, which probably represented post-translational modifications of one molecule.

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