Search: onr:"swepub:oai:DiVA.org:umu-59886" >
Cloning and charact...
-
Ny, TorUmeå universitet,Institutionen för medicinsk kemi och biofysik
(author)
Cloning and characterization of a cDNA for rat tissue-type plasminogen activator.
- Article/chapterEnglish1988
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:umu-59886
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-59886URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Two partly overlapping lambda gt11 cDNA clones coding for the 22S rat tissue-type plasminogen activator (t-PA) mRNA were isolated. The cDNA sequences cover 2445 nucleotides of the mRNA, including a 5' untranslated region of 31 nucleotides, an open reading frame of 1677 nucleotides, a 3' untranslated region of 737 nucleotides, and a poly(A) tail. The open reading frame codes for a 17-amino-acid signal peptide, a propeptide with 12 amino acids, and the mature protein with 530 amino acids. Rat t-PA has 81% and 92% amino acid sequence identity with the human and mouse counterparts and an equal distribution of conserved amino acids, suggesting that the proteins can fold into identical three-dimensional structures. The rat t-PA sequence contains two putative N-glycosylation sites at Asn-120 and Asn-452, while human t-PA has an additional glycosylation site at Asn-187. The site at Asn-187 is glycosylated in the human protein, revealing a different glycosylation pattern between the human and rat proteins.
Added entries (persons, corporate bodies, meetings, titles ...)
-
Leonardsson, G
(author)
-
Hsueh, A J
(author)
-
Umeå universitetInstitutionen för medicinsk kemi och biofysik
(creator_code:org_t)
Related titles
-
In:DNA (Mary Ann Liebert, Inc.)7:10, s. 671-70198-0238
Internet link
Find in a library
To the university's database