Search: onr:"swepub:oai:DiVA.org:umu-59911" >
Hormonal regulation...
Hormonal regulation of expression of tissue type plasminogen activator and plasminogen activator inhibitor type 1 in cultured rat granulosa cells.
- Article/chapterEnglish1994
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:umu-59911
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-59911URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
In the present study, gonadotropin and gonadotropin-releasing hormone (GnRH) regulation of tPA and PAI-1 expression in PMSG-primed granulosa cells has been investigated. (i) Addition of gonadotropins (FSH and LH) and GnRH agonist (GnRHa) or PMA to the culture increases tPA activity; FSH (or LH) plus GnRHa (or PMA) in the culture further enhances the enzyme production to such an extent that a more obvious effect than the additive effect caused by these hormones used alone has been observed; (ii) in contrast, FSH and LH decrease PAI-1 activity, whereas GnRHa and PMA alone markedly increase PAI-1 mRNA level and PAI-1 activity. Because FSH and LH stimulate tPA production and have no significant effect on PAI-1 mRNA induction, the observed inhibition of PAI-1 activity by gonadotropins may be due to the occurrence of neutralization of PA and PAI-1 proteins in the conditioned media by the formation of complexes between PA and PAI-1; (iii) increases in PAI-1 mRNA level and activity by GnRH and PMA are completely inhibited by the co-addition of FSH or LH to the culture. It is, therefore, suggested that the mechanism of gonadotropin and GnRHa regulation of tPA and PAI-1 expression in granulosa cells is different.
Added entries (persons, corporate bodies, meetings, titles ...)
-
Peng, X R
(author)
-
Liu, K
(author)
-
Ny, TorUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)tony0001
(author)
-
Umeå universitetInstitutionen för medicinsk kemi och biofysik
(creator_code:org_t)
Related titles
-
In:Science in China. Series B, Chemistry, life sciences & earth sciences37:3, s. 271-91001-652X
Internet link
Find in a library
To the university's database