Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses

van Mierlo, Joël T (author)

Bronkhorst, Alfred W (author)

Overheul, Gijs J (author)

Sadanandan, Sajna A (author): Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Hultmark

Ekström, Jens-Ola (author): Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Hultmark

Heestermans, Marco (author)

Hultmark, Dan (author): Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Institute of Biomedical Technology, University of Tampere, Tampere, Finland,Hultmark, Univ Tampere, Inst Biomed Technol, FIN-33101 Tampere, Finland

Antoniewski, Christophe (author)

van Rij, Ronald P (author)

(creator_code:org_t)

2012-08-16
2012
English.
In: PLoS Pathogens. - : Public Library of Science (PLoS). - 1553-7366 .- 1553-7374. ; 8:8

Related links:: https://doi.org/10.1...; show more...; https://umu.diva-por... (primary) (Raw object); https://journals.plo...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppressive activity in vitro and in vivo that enhances pathogenicity of recombinant Sindbis virus in an RNAi dependent manner. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. The convergent evolution of AGO2 suppression in two unrelated insect RNA viruses highlights the importance of AGO2 in antiviral defense.

By the author/editor: van Mierlo, Joël ...; Bronkhorst, Alfr ...; Overheul, Gijs J; Sadanandan, Sajn ...; Ekström, Jens-Ol ...; Heestermans, Mar ...; show more...; Hultmark, Dan; Antoniewski, Chr ...; van Rij, Ronald ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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