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Reduction in WT1 Ge...
Reduction in WT1 Gene Expression During Early Treatment Predicts the Outcome in Patients With Acute Myeloid Leukemia
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- Andersson, Charlotta (author)
- Umeå universitet,Klinisk kemi
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- Li, Xingru (author)
- Umeå universitet,Klinisk kemi
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- Lorenz, Fryderyk (author)
- Umeå universitet,Onkologi
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- Golovleva, Irina (author)
- Umeå universitet,Medicinsk och klinisk genetik
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- Wahlin, Anders (author)
- Umeå universitet,Onkologi
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- Li, Aihong (author)
- Umeå universitet,Klinisk kemi
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(creator_code:org_t)
- Lippincott Williams & Wilkins, 2012
- 2012
- English.
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In: Diagnostic molecular pathology (Print). - : Lippincott Williams & Wilkins. - 1052-9551 .- 1533-4066. ; 21:4, s. 225-233
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Wilms tumor gene 1 (WT1) expression has been suggested as an applicable minimal residual disease marker in acute myeloid leukemia (AML). We evaluated the use of this marker in 43 adult AML patients. Quantitative assessment of WT1 gene transcripts was performed using real-time quantitative-polymerase chain reaction assay. Samples from both the peripheral blood and the bone marrow were analyzed at diagnosis and during follow-up. A strong correlation was observed between WT1 normalized with 2 different control genes (beta-actin and ABL1, P < 0.001). WT1 mRNA level at diagnosis was of no prognostic relevance (P > 0.05). A >= 1-log reduction in WT1 expression in bone marrow samples taken < 1 month after diagnosis significantly correlated with an improved overall survival (P = 0.004) and freedom from relapse (P = 0.010) when beta-actin was used as control gene. Furthermore, a reduction in WT1 expression by >= 2 logs in peripheral blood samples taken at a later time point significantly correlated with a better outcome for overall survival (P = 0.004) and freedom from relapse (P = 0.012). This result was achieved when normalizing against both b-actin and ABL1. These results therefore suggest that WT1 gene expression can provide useful information for minimal residual disease detection in adult AML patients and that combined use of control genes can give more informative results.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- WT1 gene expression
- acute myeloid leukemia
- minimal residual disease
- RQ-PCR
- control genes
Publication and Content Type
- ref (subject category)
- art (subject category)
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