Sökning: onr:"swepub:oai:DiVA.org:umu-62801" >
Myeloid-related pro...
Myeloid-related protein-14 contributes to protective immunity in gram-negative pneumonia derived sepsis
-
Achouiti, Ahmed (författare)
-
Vogl, Thomas (författare)
-
- Urban, Constantin F (författare)
- Umeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS)
-
visa fler...
-
- Röhm, Marc (författare)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Klinisk bakteriologi
-
Hommes, Tijmen J (författare)
-
van Zoelen, Marieke AD (författare)
-
Florquin, Sandrine (författare)
-
Roth, Johannes (författare)
-
van't Veer, Cornelis (författare)
-
de Vos, Alex F (författare)
-
van der Poll, Tom (författare)
-
visa färre...
-
(creator_code:org_t)
- 2012-10-25
- 2012
- Engelska.
-
Ingår i: PLoS Pathogens. - San Fransisco : Public Library of Science. - 1553-7374. ; 8:10, s. e1002987-
- Relaterad länk:
-
https://umu.diva-por... (primary) (Raw object)
-
visa fler...
-
https://journals.plo...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Klebsiella (K.) pneumoniae is a common cause of pneumonia-derived sepsis. Myeloid related protein 8 (MRP8, S100A8) and MRP14 (S100A9) are the most abundant cytoplasmic proteins in neutrophils. They can form MRP8/14 heterodimers that are released upon cell stress stimuli. MRP8/14 reportedly exerts antimicrobial activity, but in acute fulminant sepsis models MRP8/14 has been found to contribute to organ damage and death. We here determined the role of MRP8/14 in K. pneumoniae sepsis originating from the lungs, using an established model characterized by gradual growth of bacteria with subsequent dissemination. Infection resulted in gradually increasing MRP8/14 levels in lungs and plasma. Mrp14 deficient (mrp14(-/-)) mice, unable to form MRP8/14 heterodimers, showed enhanced bacterial dissemination accompanied by increased organ damage and a reduced survival. Mrp14(-/-) macrophages were reduced in their capacity to phagocytose Klebsiella. In addition, recombinant MRP8/14 heterodimers, but not MRP8 or MRP14 alone, prevented growth of Klebsiella in vitro through chelation of divalent cations. Neutrophil extracellular traps (NETs) prepared from wildtype but not from mrp14(-/-) neutrophils inhibited Klebsiella growth; in accordance, the capacity of human NETs to kill Klebsiella was strongly impaired by an anti-MRP14 antibody or the addition of zinc. These results identify MRP8/14 as key player in protective innate immunity during Klebsiella pneumonia.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Achouiti, Ahmed
-
Vogl, Thomas
-
Urban, Constanti ...
-
Röhm, Marc
-
Hommes, Tijmen J
-
van Zoelen, Mari ...
-
visa fler...
-
Florquin, Sandri ...
-
Roth, Johannes
-
van't Veer, Corn ...
-
de Vos, Alex F
-
van der Poll, To ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Mikrobiologi
- Artiklar i publikationen
-
PLoS Pathogens
- Av lärosätet
-
Umeå universitet