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Molecular basis for...
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van Weering, Jan R. T.
(author)
Molecular basis for SNX-BAR-mediated assembly of distinct endosomal sorting tubules
- Article/chapterEnglish2012
Publisher, publication year, extent ...
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2012-10-19
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Wiley,2012
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-63025
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-63025URI
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https://doi.org/10.1038/emboj.2012.283DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Sorting nexins (SNXs) are regulators of endosomal sorting. For the SNX-BAR subgroup, a Bin/Amphiphysin/Rvs (BAR) domain is vital for formation/stabilization of tubular subdomains that mediate cargo recycling. Here, by analysing the in vitro membrane remodelling properties of all 12 human SNX-BARs, we report that some, but not all, can elicit the formation of tubules with diameters that resemble sorting tubules observed in cells. We reveal that SNX-BARs display a restricted pattern of BAR domain-mediated dimerization, and by resolving a 2.8 angstrom structure of a SNX1-BAR domain homodimer, establish that dimerization is achieved in part through neutralization of charged residues in the hydrophobic BAR-dimerization interface. Membrane remodelling also requires functional amphipathic helices, predicted to be present in all SNX-BARs, and the formation of high order SNX-BAR oligomers through selective 'tip-loop' interactions. Overall, the restricted and selective nature of these interactions provide a molecular explanation for how distinct SNX-BAR-decorated tubules are nucleated from the same endosomal vacuole, as observed in living cells. Our data provide insight into the molecular mechanism that generates and organizes the tubular endosomal network.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Sessions, Richard B.
(author)
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Traer, Colin J.
(author)
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Kloer, Daniel P.
(author)
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Bhatia, Vikram K.
(author)
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Stamou, Dimitrios
(author)
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Carlsson, Sven R.Umeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)svca0001
(author)
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Hurley, James H.
(author)
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Cullen, Peter J.
(author)
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Umeå universitetInstitutionen för medicinsk kemi och biofysik
(creator_code:org_t)
Related titles
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In:EMBO Journal: Wiley31:23, s. 4466-44800261-41891460-2075
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